The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide

The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide

Isolated human peripheral lymphocytes were treated in vitro with styrene-7, 8–oxide (SO) and the kinetics of the repair of induced DNA damage was assessed by comet assay during further incubation of lymphocytes. Using a modified assay we measured simultaneously the number of single strand breaks in...

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Main Authors: Renata Köhlerová, Rudolf Štětina
Format: Article
Language:English
Published: Karolinum Press 2003-01-01
Series:Acta Medica
Subjects:
Styrene oxide
SSBs
DNA repair
Human lymphocytes
Comet assay
Online Access:https://actamedica.lfhk.cuni.cz/46/3/0095/
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spelling doaj-2c4d26965f9449dcaaf93a74c490b5712020-11-24T21:41:04ZengKarolinum PressActa Medica1211-42861805-96942003-01-014639510010.14712/18059694.2019.14The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene OxideRenata Köhlerová0Rudolf Štětina1Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biochemistry, Hradec Králové, Czech RepublicPurkyně Military Medical Academy in Hradec Králové, Department of Toxicology, Hradec Králové, Czech RepublicIsolated human peripheral lymphocytes were treated in vitro with styrene-7, 8–oxide (SO) and the kinetics of the repair of induced DNA damage was assessed by comet assay during further incubation of lymphocytes. Using a modified assay we measured simultaneously the number of single strand breaks in DNA (SSBs) and the sites sensitive to endonuclease III (endo III) that most probably represent abasic sites in DNA molecules. SO induced DNA damage in a dose–dependent manner and both SSBs and endo III sites were removed from the DNA by a repair process with a half time about 2–4 hours. The damage was repaired completely within 12 hours after the treatment.https://actamedica.lfhk.cuni.cz/46/3/0095/Styrene oxideSSBsDNA repairHuman lymphocytesComet assay
collection DOAJ
language English
format Article
sources DOAJ
author Renata Köhlerová
Rudolf Štětina
spellingShingle Renata Köhlerová
Rudolf Štětina
The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide
Acta Medica
Styrene oxide
SSBs
DNA repair
Human lymphocytes
Comet assay
author_facet Renata Köhlerová
Rudolf Štětina
author_sort Renata Köhlerová
title The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide
title_short The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide
title_full The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide
title_fullStr The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide
title_full_unstemmed The Repair of DNA Damage Induced in Human Peripheral Lymphocytes with Styrene Oxide
title_sort repair of dna damage induced in human peripheral lymphocytes with styrene oxide
publisher Karolinum Press
series Acta Medica
issn 1211-4286
1805-9694
publishDate 2003-01-01
description Isolated human peripheral lymphocytes were treated in vitro with styrene-7, 8–oxide (SO) and the kinetics of the repair of induced DNA damage was assessed by comet assay during further incubation of lymphocytes. Using a modified assay we measured simultaneously the number of single strand breaks in DNA (SSBs) and the sites sensitive to endonuclease III (endo III) that most probably represent abasic sites in DNA molecules. SO induced DNA damage in a dose–dependent manner and both SSBs and endo III sites were removed from the DNA by a repair process with a half time about 2–4 hours. The damage was repaired completely within 12 hours after the treatment.
topic Styrene oxide
SSBs
DNA repair
Human lymphocytes
Comet assay
url https://actamedica.lfhk.cuni.cz/46/3/0095/
work_keys_str_mv AT renatakohlerova therepairofdnadamageinducedinhumanperipherallymphocyteswithstyreneoxide
AT rudolfstetina therepairofdnadamageinducedinhumanperipherallymphocyteswithstyreneoxide
AT renatakohlerova repairofdnadamageinducedinhumanperipherallymphocyteswithstyreneoxide
AT rudolfstetina repairofdnadamageinducedinhumanperipherallymphocyteswithstyreneoxide
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