Quantitative polymerase chain reaction: another tool to evaluate viable virus content in live attenuated orf vaccine

Quantitative polymerase chain reaction: another tool to evaluate viable virus content in live attenuated orf vaccine

A probe-based real-time polymerase chain reaction (PCR) assay based on the highly conserved DNA polymerase gene of orf virus (ORFV) for the quality control of attenuated orf vaccine is reported. Primary lamb testis (PLT) cells were infected with orf vaccine virus and harvested at a critical time poi...

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Bibliographic Details
Main Authors: Durlav Prasad Bora, Veerakyathappa Bhanuprakash, Gnanavel Venkatesan, Vinayagamurthy Balamurugan, Manimuthu Prabhu, Revaniah Yogisharadhya
Format: Article
Language:English
Published: Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale 2012-12-01
Series:Veterinaria Italiana
Subjects:
Contagious ecthyma
DNA polymerase gene
India
Orf
PCR
Polymerase chain reaction
Potency
Real-time PCR
TaqMan
Vaccine
Virus
Online Access:http://www.izs.it/vet_italiana/2012/48_4/425.pdf

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