| Summary: | In this study, we developed microsatellite markers, analyzed their distribution and frequency and verified polymorphism based on the transcriptome data of pearl oyster Pinctada maxima. Totally, 58,121 potential EST-SSRs from 145,877 sequences were identified. We randomly selected 100 of these primer pairs for further validation in the stock, 76 primer pairs were successfully amplified and 25 primer pairs were found to be polymorphic. The number of alleles at each locus ranged from 2 to 5, with an average allele number of 2.96. The observed heterozygosity ranged from 0.185 to 0.931 with an average of 0.656. The expected heterozygosity ranged from 0.337 to 0.754 with an average of 0.552. The polymorphic information content ranged from 0.263 to 0.690 with an average of 0.462. These markers will provide an effective tool for genetic diversity, genetic map construction and molecular-assisted breeding in the species of P. maxima. Keywords: Pinctada maxima, SSR markers, Polymorphism, Genetic diversity
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