Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study

Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study

Abstract Background Viral infection by dengue virus is a major public health problem in tropical countries. Early diagnosis and detection are increasingly based on quantitative reverse transcriptase real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between diff...

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Bibliographic Details
Main Authors: Kevin Vanneste, Linda Garlant, Sylvia Broeders, Steven Van Gucht, Nancy H. Roosens
Format: Article
Language:English
Published: BMC 2018-09-01
Series:BMC Bioinformatics
Subjects:
Dengue virus
RT-qPCR
BLAST
Virus detection
Online Access:http://link.springer.com/article/10.1186/s12859-018-2313-0

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