Effect of temperature and time after collection on buck sperm quality

Abstract Background Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extende...

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Main Authors: Kirsten Hahn, Klaus Failing, Axel Wehrend
Format: Article
Language:English
Published: BMC 2019-10-01
Series:BMC Veterinary Research
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12917-019-2135-y
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spelling doaj-2bd7f8e3941f4fac9e2f616638d104482020-11-25T03:40:11ZengBMCBMC Veterinary Research1746-61482019-10-011511710.1186/s12917-019-2135-yEffect of temperature and time after collection on buck sperm qualityKirsten Hahn0Klaus Failing1Axel Wehrend2Equine Hospital, Ludwig-Maximilians-UniversityUnit for Biomathematics and Data Processing, Justus-Liebig UniversityClinic for Obstetrics, Gynecology and Andrology of Large and Small Animals with Ambulatory Service, Justus-Liebig-UniversityAbstract Background Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extender. Here we examined the effects of two handling temperatures (20 °C, 37 °C) and various examination time points (3–30 min) after semen collection. Results Examination time point had a significant influence on raw sperm viability (p < 0.05), motility (p < 0.05) and on semen pH (p < 0.05). The two different handling temperatures had no significant effect on sperm viability (p > 0.05), motility (p > 0.05), with the exception of fast moving sperm (p = 0.04), or on semen pH (p > 0.05). Conclusion Examination time point was identified as factor strongly influencing raw peacock buck semen after collection. Raw goat semen can tolerate room temperatures for at least 10 min without impacting overall semen quality. In order to obtain comparable results, semen samples should always be examined within 10 min after collection.http://link.springer.com/article/10.1186/s12917-019-2135-yPeacock bucksTemperatureTime pointComputer assisted semen analysis
collection DOAJ
language English
format Article
sources DOAJ
author Kirsten Hahn
Klaus Failing
Axel Wehrend
spellingShingle Kirsten Hahn
Klaus Failing
Axel Wehrend
Effect of temperature and time after collection on buck sperm quality
BMC Veterinary Research
Peacock bucks
Temperature
Time point
Computer assisted semen analysis
author_facet Kirsten Hahn
Klaus Failing
Axel Wehrend
author_sort Kirsten Hahn
title Effect of temperature and time after collection on buck sperm quality
title_short Effect of temperature and time after collection on buck sperm quality
title_full Effect of temperature and time after collection on buck sperm quality
title_fullStr Effect of temperature and time after collection on buck sperm quality
title_full_unstemmed Effect of temperature and time after collection on buck sperm quality
title_sort effect of temperature and time after collection on buck sperm quality
publisher BMC
series BMC Veterinary Research
issn 1746-6148
publishDate 2019-10-01
description Abstract Background Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extender. Here we examined the effects of two handling temperatures (20 °C, 37 °C) and various examination time points (3–30 min) after semen collection. Results Examination time point had a significant influence on raw sperm viability (p < 0.05), motility (p < 0.05) and on semen pH (p < 0.05). The two different handling temperatures had no significant effect on sperm viability (p > 0.05), motility (p > 0.05), with the exception of fast moving sperm (p = 0.04), or on semen pH (p > 0.05). Conclusion Examination time point was identified as factor strongly influencing raw peacock buck semen after collection. Raw goat semen can tolerate room temperatures for at least 10 min without impacting overall semen quality. In order to obtain comparable results, semen samples should always be examined within 10 min after collection.
topic Peacock bucks
Temperature
Time point
Computer assisted semen analysis
url http://link.springer.com/article/10.1186/s12917-019-2135-y
work_keys_str_mv AT kirstenhahn effectoftemperatureandtimeaftercollectiononbuckspermquality
AT klausfailing effectoftemperatureandtimeaftercollectiononbuckspermquality
AT axelwehrend effectoftemperatureandtimeaftercollectiononbuckspermquality
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