Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study

Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study

Our research objective was to develop novel drug delivery vehicles consisting of TiO<sub>2</sub> and Al<sub>2</sub>O<sub>3</sub> nanoparticles encapsulated by a bilayer shell that allows the reversible embedment of hydrophobic drugs. The first shell is formed by c...

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Main Authors: Stefanie Klein, Tobias Luchs, Andreas Leng, Luitpold V. R. Distel, Winfried Neuhuber, Andreas Hirsch
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:Bioengineering
Subjects:
shell-by-shell nanoparticles
TiO<sub>2</sub> nanoparticles
Al<sub>2</sub>O<sub>3 </sub>nanoparticles
quercetin
drug delivery
radiotherapy
Online Access:https://www.mdpi.com/2306-5354/7/4/126
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spelling doaj-2bbd3d0b60e148df89c771e0005fb2232020-11-25T03:59:17ZengMDPI AGBioengineering2306-53542020-10-01712612610.3390/bioengineering7040126Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro StudyStefanie Klein0Tobias Luchs1Andreas Leng2Luitpold V. R. Distel3Winfried Neuhuber4Andreas Hirsch5Department of Chemistry and Pharmacy, Physical Chemistry I and ICMM, Friedrich-Alexander University of Erlangen Nuremberg, Egerlandstr. 3, D-91058 Erlangen, GermanyDepartment of Chemistry and Pharmacy, Chair of Organic Chemistry II, Friedrich-Alexander University of Erlangen Nuremberg, Nikolaus-Fiebiger-Str. 10, D-91058 Erlangen, GermanyDepartment of Chemistry and Pharmacy, Chair of Organic Chemistry II, Friedrich-Alexander University of Erlangen Nuremberg, Nikolaus-Fiebiger-Str. 10, D-91058 Erlangen, GermanyDepartment of Radiation Oncology, Friedrich-Alexander University of Erlangen Nuremberg, Universitätsstr. 27, D-91054 Erlangen, GermanyInstitute of Anatomy and Cell Biology, Chair of Anatomy I, Friedrich-Alexander University of Erlangen Nuremberg, Krankenhausstr. 9, D-91054 Erlangen, GermanyDepartment of Chemistry and Pharmacy, Chair of Organic Chemistry II, Friedrich-Alexander University of Erlangen Nuremberg, Nikolaus-Fiebiger-Str. 10, D-91058 Erlangen, GermanyOur research objective was to develop novel drug delivery vehicles consisting of TiO<sub>2</sub> and Al<sub>2</sub>O<sub>3</sub> nanoparticles encapsulated by a bilayer shell that allows the reversible embedment of hydrophobic drugs. The first shell is formed by covalent binding of hydrophobic phosphonic acid at the metal oxide surface. The second shell composed of amphiphilic sodium dodecylbenzenesulfonate emerges by self-aggregation driven by hydrophobic interactions between the dodecylbenzene moiety and the hydrophobic first shell. The resulting double layer provides hydrophobic pockets suited for the intake of hydrophobic drugs. The nanoparticles were loaded with the anticancer drugs quercetin and 7-amino-4-methylcoumarin. Irradiation with X-rays was observed to release the potential anticancer drugs into the cytoplasm. In Michigan Cancer Foundation (MCF)-10 A cells, quercetin and 7-amino-4-methylcoumarin acted as antioxidants by protecting the non-tumorigenic cells from harmful radiation effects. In contrast, these agents increased the reactive oxygen species (ROS) formation in cancerous MCF-7 cells. Quercetin and 7-amino-4-methylcoumarin were shown to induce apoptosis via the mitochondrial pathway in cancer cells by determining an increase in TUNEL-positive cells and a decrease in mitochondrial membrane potential after irradiation. After X-ray irradiation, the survival fraction of MCF-7 cells with drug-loaded nanoparticles considerably decreased, which demonstrates the excellent performance of the double-layer stabilized nanoparticles as drug delivery vehicles.https://www.mdpi.com/2306-5354/7/4/126shell-by-shell nanoparticlesTiO<sub>2</sub> nanoparticlesAl<sub>2</sub>O<sub>3 </sub>nanoparticlesquercetindrug deliveryradiotherapy
collection DOAJ
language English
format Article
sources DOAJ
author Stefanie Klein
Tobias Luchs
Andreas Leng
Luitpold V. R. Distel
Winfried Neuhuber
Andreas Hirsch
spellingShingle Stefanie Klein
Tobias Luchs
Andreas Leng
Luitpold V. R. Distel
Winfried Neuhuber
Andreas Hirsch
Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study
Bioengineering
shell-by-shell nanoparticles
TiO<sub>2</sub> nanoparticles
Al<sub>2</sub>O<sub>3 </sub>nanoparticles
quercetin
drug delivery
radiotherapy
author_facet Stefanie Klein
Tobias Luchs
Andreas Leng
Luitpold V. R. Distel
Winfried Neuhuber
Andreas Hirsch
author_sort Stefanie Klein
title Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study
title_short Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study
title_full Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study
title_fullStr Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study
title_full_unstemmed Encapsulation of Hydrophobic Drugs in Shell-by-Shell Coated Nanoparticles for Radio—and Chemotherapy—An In Vitro Study
title_sort encapsulation of hydrophobic drugs in shell-by-shell coated nanoparticles for radio—and chemotherapy—an in vitro study
publisher MDPI AG
series Bioengineering
issn 2306-5354
publishDate 2020-10-01
description Our research objective was to develop novel drug delivery vehicles consisting of TiO<sub>2</sub> and Al<sub>2</sub>O<sub>3</sub> nanoparticles encapsulated by a bilayer shell that allows the reversible embedment of hydrophobic drugs. The first shell is formed by covalent binding of hydrophobic phosphonic acid at the metal oxide surface. The second shell composed of amphiphilic sodium dodecylbenzenesulfonate emerges by self-aggregation driven by hydrophobic interactions between the dodecylbenzene moiety and the hydrophobic first shell. The resulting double layer provides hydrophobic pockets suited for the intake of hydrophobic drugs. The nanoparticles were loaded with the anticancer drugs quercetin and 7-amino-4-methylcoumarin. Irradiation with X-rays was observed to release the potential anticancer drugs into the cytoplasm. In Michigan Cancer Foundation (MCF)-10 A cells, quercetin and 7-amino-4-methylcoumarin acted as antioxidants by protecting the non-tumorigenic cells from harmful radiation effects. In contrast, these agents increased the reactive oxygen species (ROS) formation in cancerous MCF-7 cells. Quercetin and 7-amino-4-methylcoumarin were shown to induce apoptosis via the mitochondrial pathway in cancer cells by determining an increase in TUNEL-positive cells and a decrease in mitochondrial membrane potential after irradiation. After X-ray irradiation, the survival fraction of MCF-7 cells with drug-loaded nanoparticles considerably decreased, which demonstrates the excellent performance of the double-layer stabilized nanoparticles as drug delivery vehicles.
topic shell-by-shell nanoparticles
TiO<sub>2</sub> nanoparticles
Al<sub>2</sub>O<sub>3 </sub>nanoparticles
quercetin
drug delivery
radiotherapy
url https://www.mdpi.com/2306-5354/7/4/126
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AT tobiasluchs encapsulationofhydrophobicdrugsinshellbyshellcoatednanoparticlesforradioandchemotherapyaninvitrostudy
AT andreasleng encapsulationofhydrophobicdrugsinshellbyshellcoatednanoparticlesforradioandchemotherapyaninvitrostudy
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AT winfriedneuhuber encapsulationofhydrophobicdrugsinshellbyshellcoatednanoparticlesforradioandchemotherapyaninvitrostudy
AT andreashirsch encapsulationofhydrophobicdrugsinshellbyshellcoatednanoparticlesforradioandchemotherapyaninvitrostudy
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