Integration and exchange of split dCas9 domains for transcriptional controls in mammalian cells
Molecular engineering of Cas9 has the potential to expand the application of CRISPR-Cas technology. Here, Ma et al. show that dCas9 can be split and reconstituted in human cells and use a domain swapping strategy to engineer custom Cas9-based logic circuits and sensory switches.
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2016-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms13056 |