| Summary: | Metabolomics is beginning to be used for assessing unintended changes in genetically modified (GM) crops. To investigate whether<i> Cry1C </i>gene transformation would induce metabolic changes in rice plants, and whether the metabolic changes would pose potential risks when <i>Cry1C </i>rice plants are exposed to rice dwarf virus (RDV), the metabolic profiles of <i>Cry1C</i> rice T1C-19 and its non-<i>Bt </i>parental rice MH63 under RDV-free and RDV-infected status were analyzed using gas chromatography–mass spectrometry (GC-MS). Compared to MH63 rice, slice difference was detected in T1C-19 under RDV-free conditions (less than 3%), while much more metabolites showed significant response to RDV infection in T1C-19 (15.6%) and in MH63 (5.0%). Pathway analysis showed biosynthesis of lysine, valine, leucine, and isoleucine may be affected by RDV infection in T1C-19. No significant difference in the contents of free amino acids (AAs) was found between T1C-19 and MH63 rice, and the free AA contents of the two rice plants showed similar responses to RDV infection. Furthermore, no significant differences of the RDV infection rates between T1C-19 and MH63 were detected. Our results showed the <i>Cry1C </i>gene transformation did not affect the sensitivity of rice to RDV, indicating <i>Cry1C</i> rice would not aggravate the epidemic and dispersal of RDV.
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