Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus
The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locu...
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| Online Access: | https://peerj.com/articles/731.pdf |
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doaj-2b46679f9bd248858f48437cc959e3052020-11-24T23:13:42ZengPeerJ Inc.PeerJ2167-83592015-01-013e73110.7717/peerj.731731Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylusCarla Sousa-Santos0Paulo J. Fonseca1Maria Clara P. Amorim2MARE—Marine and Environmental Sciences Centre, ISPA—Instituto Universitário, Rua Jardim do Tabaco, Lisbon, PortugalDepartamento de Biologia Animal and Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Lisbon, PortugalMARE—Marine and Environmental Sciences Centre, ISPA—Instituto Universitário, Rua Jardim do Tabaco, Lisbon, PortugalThe Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy–Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species.https://peerj.com/articles/731.pdfBatrachoididaePaternityPolymorphic lociMicrosatellite developmentPyrosequencing |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Carla Sousa-Santos Paulo J. Fonseca Maria Clara P. Amorim |
| spellingShingle |
Carla Sousa-Santos Paulo J. Fonseca Maria Clara P. Amorim Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus PeerJ Batrachoididae Paternity Polymorphic loci Microsatellite development Pyrosequencing |
| author_facet |
Carla Sousa-Santos Paulo J. Fonseca Maria Clara P. Amorim |
| author_sort |
Carla Sousa-Santos |
| title |
Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus |
| title_short |
Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus |
| title_full |
Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus |
| title_fullStr |
Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus |
| title_full_unstemmed |
Development and characterization of novel microsatellite loci for Lusitanian toadfish, Halobatrachus didactylus |
| title_sort |
development and characterization of novel microsatellite loci for lusitanian toadfish, halobatrachus didactylus |
| publisher |
PeerJ Inc. |
| series |
PeerJ |
| issn |
2167-8359 |
| publishDate |
2015-01-01 |
| description |
The Lusitanian toadfish Halobatrachus didactylus is an eastern Atlantic polygynous species showing male paternal care. In this paper we describe 5 novel microsatellite loci obtained by 454 GS-FLX Titanium pyrosequencing of a microsatellite-enriched library. The number of alleles per polymorphic locus varied between 2 and 4, and the observed heterozygosity ranged from 0.082 to 0.600. No significant deviation from Hardy–Weinberg equilibrium was found and there was no evidence for linkage disequilibrium. These markers will be of great value for paternity studies and population genetics of this species. |
| topic |
Batrachoididae Paternity Polymorphic loci Microsatellite development Pyrosequencing |
| url |
https://peerj.com/articles/731.pdf |
| work_keys_str_mv |
AT carlasousasantos developmentandcharacterizationofnovelmicrosatellitelociforlusitaniantoadfishhalobatrachusdidactylus AT paulojfonseca developmentandcharacterizationofnovelmicrosatellitelociforlusitaniantoadfishhalobatrachusdidactylus AT mariaclarapamorim developmentandcharacterizationofnovelmicrosatellitelociforlusitaniantoadfishhalobatrachusdidactylus |
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