Screening of specific diagnostic peptides of swine hepatitis E virus

• Main Authors: Zhu Hong, Li Jianyue, Li Zhen, Yu Ruisong, Liu Qiwen, Zhao Kai, Wu Xiao, Tan Furong, Wang Jinbin, Tang Xueming
• Format: Article
• Language: English
• Published: BMC 2009-11-01
• Series: Virology Journal
• Online Access: http://www.virologyj.com/content/6/1/186

<p>Abstract</p> <p>Background</p> <p>Swine hepatitis E virus (swHEV) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health. Elucidation of the major antigenic epitopes of swHEV is essential for the effective control of swHEV epidemics.</p> <p>Results</p> <p>By bioinformatic analysis, we identified and then synthesized 12 peptides from open reading frames (ORFs) ORF1, ORF2 and ORF3, including swHEV-1 - swHEV-12. Using the results from ELISA, we selected swHEV-11 as the best candidate antigen and used it as a coating antigen for the development of peptide-based swine anti-HEV ELISA kits. The coefficient of variation (CV) the coefficient of variation (CV) varied between 4.3-7.2% in the same batch, and between 8.2-17.7% in six different batches. When comparing our swine peptide-based kit with the commercial recombinant-based kit, the humane anti-HEV IgG test had a 73.4% correspondence rate for them.</p> <p>Conclusion</p> <p>This is the first systemic study to screen the diagnostic peptides of swHEV and our findings strongly suggest that peptide swHEV-11 is a potent diagnostic reagent of swHEV that could be used in the development of highly efficient diagnostic assays for the specific and highly sensitive detection of anti-HEV activity in swine serum samples.</p>