A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2

In this study, the most suitable vegetable extract was screened to use as non-conventional nutrient sources for cellulose production of Rhodococcus sp. MI 2. SH medium or a synthetic medium was used as a conventional or control medium. Cha-poo (Piper sarmentosum Roxb.) and sweet potato (Ipomoea bata...

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Main Authors: Somporn - Tanskul, Tassanaporn - Damthongsen, Nathakan - Jaturonlak
Format: Article
Language:English
Published: Universidade Federal de Uberlândia 2018-05-01
Series:Bioscience Journal
Subjects:
Online Access:http://www.seer.ufu.br/index.php/biosciencejournal/article/view/36954
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spelling doaj-2ae576b1bcaf4fd5b5a35a1389d321422021-06-29T13:20:58ZengUniversidade Federal de UberlândiaBioscience Journal1981-31632018-05-0134310.14393/BJ-v34n3a2018-3695436954A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2Somporn - Tanskul0Tassanaporn - Damthongsen1Nathakan - Jaturonlak2Prince of Songkla UniversityPrince of Songkla UniversityPrince of Songkla UniversityIn this study, the most suitable vegetable extract was screened to use as non-conventional nutrient sources for cellulose production of Rhodococcus sp. MI 2. SH medium or a synthetic medium was used as a conventional or control medium. Cha-poo (Piper sarmentosum Roxb.) and sweet potato (Ipomoea batatas Lam.) were 2 out of 14 vegetable extracts chosen as medium supplements. Rhodococcus sp. MI 2 gave the highest cellulose yield in a medium supplemented with Cha-poo extract. The optimum culture conditions in the medium supplemented with Cha-poo extract at room temperature (25o C) under static condition were 5% (v v-1) inoculum size, a 6 -day -incubation period, pH 3, 3% sucrose, and 0.5% (NH4)2SO4. The cellulose yield in the medium supplemented with Cha-poo extract was increased about 3 times (6.83 g L-1 during 6 days) higher than that obtained before optimizing (2.39 g L-1 during 6 days). The medium supplemented with Cha-poo extract cost one quarter (0.5 USD L-1 of medium) of the SH medium (1.9 USD L-1 of medium). The structure of the microfibrils of cellulose produced by Rhodococcus sp. MI 2 in a medium supplemented with Cha-poo extract observed by SEM had larger, less crowded fibrils than those produced in the medium supplemented with sweet potato extract. In addition, the microfibrils of the former had many beehive shaped knots whereas those of the latter had mantle-like surrounding the fibrils.http://www.seer.ufu.br/index.php/biosciencejournal/article/view/36954bacterial cellulosecha-poopiper sarmentosumsweet potatoipomoea batatas
collection DOAJ
language English
format Article
sources DOAJ
author Somporn - Tanskul
Tassanaporn - Damthongsen
Nathakan - Jaturonlak
spellingShingle Somporn - Tanskul
Tassanaporn - Damthongsen
Nathakan - Jaturonlak
A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
Bioscience Journal
bacterial cellulose
cha-poo
piper sarmentosum
sweet potato
ipomoea batatas
author_facet Somporn - Tanskul
Tassanaporn - Damthongsen
Nathakan - Jaturonlak
author_sort Somporn - Tanskul
title A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
title_short A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
title_full A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
title_fullStr A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
title_full_unstemmed A medium supplemented with vegetable extract used for cellulose production of Rhodococcus SP. MI 2
title_sort medium supplemented with vegetable extract used for cellulose production of rhodococcus sp. mi 2
publisher Universidade Federal de Uberlândia
series Bioscience Journal
issn 1981-3163
publishDate 2018-05-01
description In this study, the most suitable vegetable extract was screened to use as non-conventional nutrient sources for cellulose production of Rhodococcus sp. MI 2. SH medium or a synthetic medium was used as a conventional or control medium. Cha-poo (Piper sarmentosum Roxb.) and sweet potato (Ipomoea batatas Lam.) were 2 out of 14 vegetable extracts chosen as medium supplements. Rhodococcus sp. MI 2 gave the highest cellulose yield in a medium supplemented with Cha-poo extract. The optimum culture conditions in the medium supplemented with Cha-poo extract at room temperature (25o C) under static condition were 5% (v v-1) inoculum size, a 6 -day -incubation period, pH 3, 3% sucrose, and 0.5% (NH4)2SO4. The cellulose yield in the medium supplemented with Cha-poo extract was increased about 3 times (6.83 g L-1 during 6 days) higher than that obtained before optimizing (2.39 g L-1 during 6 days). The medium supplemented with Cha-poo extract cost one quarter (0.5 USD L-1 of medium) of the SH medium (1.9 USD L-1 of medium). The structure of the microfibrils of cellulose produced by Rhodococcus sp. MI 2 in a medium supplemented with Cha-poo extract observed by SEM had larger, less crowded fibrils than those produced in the medium supplemented with sweet potato extract. In addition, the microfibrils of the former had many beehive shaped knots whereas those of the latter had mantle-like surrounding the fibrils.
topic bacterial cellulose
cha-poo
piper sarmentosum
sweet potato
ipomoea batatas
url http://www.seer.ufu.br/index.php/biosciencejournal/article/view/36954
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