A Convenient, Rapid, Sensitive, and Reliable Spectrophotometric Assay for Adenylate Kinase Activity

A Convenient, Rapid, Sensitive, and Reliable Spectrophotometric Assay for Adenylate Kinase Activity

Enzymatic activity assays are essential and critical for the study of enzyme kinetics. Adenylate kinase (Adk) plays a fundamental role in cellular energy and nucleotide homeostasis. To date, assays based on different principles have been used for the determination of Adk activity. Here, we show a sp...

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Bibliographic Details
Main Authors: Kai Song, Yejing Wang, Yu Li, Chaoxiang Ding, Rui Cai, Gang Tao, Ping Zhao, Qingyou Xia, Huawei He
Format: Article
Language:English
Published: MDPI AG 2019-02-01
Series:Molecules
Subjects:
enzymatic activity assay
adenylate kinase
spectrophotometry
orthogonal experiment
bromothymol blue
Online Access:https://www.mdpi.com/1420-3049/24/4/663

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