Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium

• Main Authors: Tetsuya Muramoto, Hoshie Iriki, Jun Watanabe, Takefumi Kawata
• Format: Article
• Language: English
• Published: MDPI AG 2019-01-01
• Series: Cells
• Subjects: CRISPR/Cas9; CRISPR vector; Dictyostelium; genome editing; tRNA-based expression; Amoebozoa
• Online Access: http://www.mdpi.com/2073-4409/8/1/46

In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium.