The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
<p>Abstract</p> <p>Background</p> <p>Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been...
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doaj-2a9b2ea6d7db4d7da2b386e974709f112020-11-24T23:48:54ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662009-05-012816410.1186/1756-9966-28-64The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamsterWen Yu-mingCao Yu-tangYang SenLi AnGong Ren-guoWang Xiao-yiYu TaoWang Chang-meiYi Xin-zhu<p>Abstract</p> <p>Background</p> <p>Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been done to compare the microRNA expression patterns in oral cancer. In this study, we constructed an animal model of oral squamous cell carcinoma to investigate expression profiles of microRNAs in oral carcinogenesis.</p> <p>Methods</p> <p>The animal model of oral squamous cell carcinoma was conducted by tri-weekly (Monday, Wednesday, and Friday) painting with 5% DMBA in acetone. Six Syrian hamsters, including three from the treated group and three from the control group, were used as a training group for microRNA microarray analysis. All microarray data were analyzed by Significance Analysis of Microarrays (SAM) and CLUSTER 3.0 software, and this result was further confirmed by qRT-PCR assay.</p> <p>Results</p> <p>Seventeen microRNAs were differentially expressed in oral squamous cell carcinoma. Five microRNAs (hsa-miR-21, hsa-miR-200b, hsa-miR-221, hsa-miR-338, and mmu-miR-762) were significantly upregulated and twelve microRNAs (hsa-miR-16, hsa-miR-26a, hsa-miR-29a, hsa-miR-124a, hsa-miR-125b, mmu-miR-126-5p, hsa-miR-143, hsa-miR-145, hsa-miR-148b, hsa-miR-155, hsa-miR-199a, and hsa-miR-203) were down-regulated in cancer tissues. The expression levels of hsa-miR-21 and hsa-miR-16 seen with Stem-loop qRT-PCR were also seen in microarray analysis in all samples.</p> <p>Conclusion</p> <p>Our findings identified specific microRNA expression in oral squamous cell carcinoma and suggested that microRNAs have a role in oral carcinogenesis.</p> http://www.jeccr.com/content/28/1/64 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen Yu-ming Cao Yu-tang Yang Sen Li An Gong Ren-guo Wang Xiao-yi Yu Tao Wang Chang-mei Yi Xin-zhu |
spellingShingle |
Wen Yu-ming Cao Yu-tang Yang Sen Li An Gong Ren-guo Wang Xiao-yi Yu Tao Wang Chang-mei Yi Xin-zhu The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster Journal of Experimental & Clinical Cancer Research |
author_facet |
Wen Yu-ming Cao Yu-tang Yang Sen Li An Gong Ren-guo Wang Xiao-yi Yu Tao Wang Chang-mei Yi Xin-zhu |
author_sort |
Wen Yu-ming |
title |
The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster |
title_short |
The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster |
title_full |
The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster |
title_fullStr |
The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster |
title_full_unstemmed |
The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster |
title_sort |
expression profile of micrornas in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in syrian hamster |
publisher |
BMC |
series |
Journal of Experimental & Clinical Cancer Research |
issn |
1756-9966 |
publishDate |
2009-05-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been done to compare the microRNA expression patterns in oral cancer. In this study, we constructed an animal model of oral squamous cell carcinoma to investigate expression profiles of microRNAs in oral carcinogenesis.</p> <p>Methods</p> <p>The animal model of oral squamous cell carcinoma was conducted by tri-weekly (Monday, Wednesday, and Friday) painting with 5% DMBA in acetone. Six Syrian hamsters, including three from the treated group and three from the control group, were used as a training group for microRNA microarray analysis. All microarray data were analyzed by Significance Analysis of Microarrays (SAM) and CLUSTER 3.0 software, and this result was further confirmed by qRT-PCR assay.</p> <p>Results</p> <p>Seventeen microRNAs were differentially expressed in oral squamous cell carcinoma. Five microRNAs (hsa-miR-21, hsa-miR-200b, hsa-miR-221, hsa-miR-338, and mmu-miR-762) were significantly upregulated and twelve microRNAs (hsa-miR-16, hsa-miR-26a, hsa-miR-29a, hsa-miR-124a, hsa-miR-125b, mmu-miR-126-5p, hsa-miR-143, hsa-miR-145, hsa-miR-148b, hsa-miR-155, hsa-miR-199a, and hsa-miR-203) were down-regulated in cancer tissues. The expression levels of hsa-miR-21 and hsa-miR-16 seen with Stem-loop qRT-PCR were also seen in microarray analysis in all samples.</p> <p>Conclusion</p> <p>Our findings identified specific microRNA expression in oral squamous cell carcinoma and suggested that microRNAs have a role in oral carcinogenesis.</p> |
url |
http://www.jeccr.com/content/28/1/64 |
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