The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster

<p>Abstract</p> <p>Background</p> <p>Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been...

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Main Authors: Wen Yu-ming, Cao Yu-tang, Yang Sen, Li An, Gong Ren-guo, Wang Xiao-yi, Yu Tao, Wang Chang-mei, Yi Xin-zhu
Format: Article
Language:English
Published: BMC 2009-05-01
Series:Journal of Experimental & Clinical Cancer Research
Online Access:http://www.jeccr.com/content/28/1/64
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spelling doaj-2a9b2ea6d7db4d7da2b386e974709f112020-11-24T23:48:54ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662009-05-012816410.1186/1756-9966-28-64The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamsterWen Yu-mingCao Yu-tangYang SenLi AnGong Ren-guoWang Xiao-yiYu TaoWang Chang-meiYi Xin-zhu<p>Abstract</p> <p>Background</p> <p>Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been done to compare the microRNA expression patterns in oral cancer. In this study, we constructed an animal model of oral squamous cell carcinoma to investigate expression profiles of microRNAs in oral carcinogenesis.</p> <p>Methods</p> <p>The animal model of oral squamous cell carcinoma was conducted by tri-weekly (Monday, Wednesday, and Friday) painting with 5% DMBA in acetone. Six Syrian hamsters, including three from the treated group and three from the control group, were used as a training group for microRNA microarray analysis. All microarray data were analyzed by Significance Analysis of Microarrays (SAM) and CLUSTER 3.0 software, and this result was further confirmed by qRT-PCR assay.</p> <p>Results</p> <p>Seventeen microRNAs were differentially expressed in oral squamous cell carcinoma. Five microRNAs (hsa-miR-21, hsa-miR-200b, hsa-miR-221, hsa-miR-338, and mmu-miR-762) were significantly upregulated and twelve microRNAs (hsa-miR-16, hsa-miR-26a, hsa-miR-29a, hsa-miR-124a, hsa-miR-125b, mmu-miR-126-5p, hsa-miR-143, hsa-miR-145, hsa-miR-148b, hsa-miR-155, hsa-miR-199a, and hsa-miR-203) were down-regulated in cancer tissues. The expression levels of hsa-miR-21 and hsa-miR-16 seen with Stem-loop qRT-PCR were also seen in microarray analysis in all samples.</p> <p>Conclusion</p> <p>Our findings identified specific microRNA expression in oral squamous cell carcinoma and suggested that microRNAs have a role in oral carcinogenesis.</p> http://www.jeccr.com/content/28/1/64
collection DOAJ
language English
format Article
sources DOAJ
author Wen Yu-ming
Cao Yu-tang
Yang Sen
Li An
Gong Ren-guo
Wang Xiao-yi
Yu Tao
Wang Chang-mei
Yi Xin-zhu
spellingShingle Wen Yu-ming
Cao Yu-tang
Yang Sen
Li An
Gong Ren-guo
Wang Xiao-yi
Yu Tao
Wang Chang-mei
Yi Xin-zhu
The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
Journal of Experimental & Clinical Cancer Research
author_facet Wen Yu-ming
Cao Yu-tang
Yang Sen
Li An
Gong Ren-guo
Wang Xiao-yi
Yu Tao
Wang Chang-mei
Yi Xin-zhu
author_sort Wen Yu-ming
title The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
title_short The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
title_full The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
title_fullStr The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
title_full_unstemmed The expression profile of microRNAs in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in Syrian hamster
title_sort expression profile of micrornas in a model of 7,12-dimethyl-benz[<it>a</it>]anthrance-induced oral carcinogenesis in syrian hamster
publisher BMC
series Journal of Experimental & Clinical Cancer Research
issn 1756-9966
publishDate 2009-05-01
description <p>Abstract</p> <p>Background</p> <p>Non-coding RNA molecules, such as microRNAs, may play an important role in carcinogenesis. Recent studies have indicated that microRNAs are involved in initiation and progression of various malignancies. However, little work has been done to compare the microRNA expression patterns in oral cancer. In this study, we constructed an animal model of oral squamous cell carcinoma to investigate expression profiles of microRNAs in oral carcinogenesis.</p> <p>Methods</p> <p>The animal model of oral squamous cell carcinoma was conducted by tri-weekly (Monday, Wednesday, and Friday) painting with 5% DMBA in acetone. Six Syrian hamsters, including three from the treated group and three from the control group, were used as a training group for microRNA microarray analysis. All microarray data were analyzed by Significance Analysis of Microarrays (SAM) and CLUSTER 3.0 software, and this result was further confirmed by qRT-PCR assay.</p> <p>Results</p> <p>Seventeen microRNAs were differentially expressed in oral squamous cell carcinoma. Five microRNAs (hsa-miR-21, hsa-miR-200b, hsa-miR-221, hsa-miR-338, and mmu-miR-762) were significantly upregulated and twelve microRNAs (hsa-miR-16, hsa-miR-26a, hsa-miR-29a, hsa-miR-124a, hsa-miR-125b, mmu-miR-126-5p, hsa-miR-143, hsa-miR-145, hsa-miR-148b, hsa-miR-155, hsa-miR-199a, and hsa-miR-203) were down-regulated in cancer tissues. The expression levels of hsa-miR-21 and hsa-miR-16 seen with Stem-loop qRT-PCR were also seen in microarray analysis in all samples.</p> <p>Conclusion</p> <p>Our findings identified specific microRNA expression in oral squamous cell carcinoma and suggested that microRNAs have a role in oral carcinogenesis.</p>
url http://www.jeccr.com/content/28/1/64
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