Apoptosis inhibitory effect of anti-miR-146b on hippocampal neurons of OGD/R-induced injury

• Main Authors: Xue-Hua Li, Ying Wang, Guang-Da Sun
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2018-10-01
• Series: Journal of Hainan Medical University
• Subjects: Anti-miR-146b; Oxygen-glucose deprivation and reoxygenation; Hippocampal neurons; Apoptosis
• Online Access: http://www.hnykdxxb.com/PDF/201820/01.pdf

Objective: To construct a cellular model of oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury in hippocampal neurons in vitro, and to explore the inhibitory effect of anti-miR-146b on the apoptosis of hippocampal neurons of ischemic/reperfusion injury. Methods: Primary rat hippocampal neurons were cultured, and oxygen-glucose deprivation and reoxygenation (OGD/R) were constructed. The anti-miR-146b lentivirus was used to transfect the hippocampal neurons, and the cells were divided into the normal group, the OGD/ R group and the OGD/R + anti-miR-146b group. Real time fluorescence quantitative PCR (qRT-PCR) was used to detect the mRNA expression of miR-146b, KLF7, Caspase-3, Bcl-2 and Bax in each group. Cell viability was detected by CCK8 assay, and apoptosis was detected by Hoechst33342/PI double staining. Results: Compared with the normal group, the cell morphology of the OGD/R group and the OGD/R + anti-miR-146b group was damaged, the cell activity was decreased, and the cell apoptosis rate was increased significantly (P<0.05). However, Compared with OGD/R group, the cell morphology of the OGD/R + KLF7 group was improved, the cell activity was increased, and the apoptosis rate was decreased significantly (P<0.05). Compared with the normal group, the mRNA expression levels of miR-146b, KLF7, Caspase-3, Bax and Bcl-2 in the OGD/R group and the OGD/R + KLF7 group was increased (P<0.05). Compared with the OGD/R group, the mRNA expression of KLF7, Bcl-2 were increased in the OGD/R + KLF7 group significantly, however, the mRNA expression of miR-146b, Caspase-3, Bax were decreased significantly (P<0.05). Conclusion: Anti-miR-146b play protective effect on hippocampal neurons of OGD/R-induced injury. The mechanism may be related to upregulation of KLF7 signaling pathway, downregulation expression of Caspase-3 and Bax, upregulation expression of Bcl-2, and inhibition of apoptosis induced by OGD/R.