The Growth and Cultivation of Meristem From Shallot (Allium ascalonicum L.) cv. Katumi by In Vitro

• Main Authors: Lamro Purba, Erni Suminar, Denny Sobardini, Wieny Rizky, Syariful Mubarok
• Format: Article
• Language: English
• Published: Universitas Islam Negeri (UIN) Sunan Gunung Djati Bandung 2017-12-01
• Series: Jurnal Agro
• Subjects: Allium ascalonicum L; In Vitro; Plant Growth Regulators
• Online Access: http://journal.uinsgd.ac.id/index.php/ja/article/view/1344

<p>This study aimed for knowing and obtaining the best concentration of kinetin and NAA interaction effects in influencing the shoot induction, knowing how the plant growth regulators in induction mediastill affect the shoot additionin the MS₀media and also knowing the largest number of roots in rooting media for shallot by <em>in vitro</em>. The experiment was conducted at Laboratory of Tissue Culture Seed Technology, Faculty of Agriculture, Padjadjaran University, during January 2011 until May 2011. This experiment divided in 3 stages, namely shoot induction stage, shoot subculture to MS₀media stage and shoot subculture to rooting media stage. Experimental method used in the shoot induction stage was factorial Completely Randomized Design with three replications. The first factor was the kinetin with four levels,0, 1, 2, and 3 mg L^-1. The second factor was the NAA with three levels, as 0, 0.01, and 0.1 mg L^-1. Basic media used for each treatment was MS. The experiment result showed there was an interaction between kinetin and NAA on shoot induction stagewith the plantlet height, leaf number, and shoot addition. The best result for leaf number was gained from interaction with 2 mg L^-1kinetin without NAA,while the treatment of 2 mg L^-1kinetin with 0.01 mg L^-1NAA gave a better interaction for theshoot addition variable.</p>