A ribosomal misincorporation of Lys for Arg in human triosephosphate isomerase expressed in Escherichia coli gives rise to two protein populations.

A ribosomal misincorporation of Lys for Arg in human triosephosphate isomerase expressed in Escherichia coli gives rise to two protein populations.

We previously observed that human homodimeric triosephosphate isomerase (HsTIM) expressed in Escherichia coli and purified to apparent homogeneity exhibits two significantly different thermal transitions. A detailed exploration of the phenomenon showed that the preparations contain two proteins; one...

Full description

Bibliographic Details
Main Authors: Beatriz Aguirre, Miguel Costas, Nallely Cabrera, Guillermo Mendoza-Hernández, Donald L Helseth, Paulette Fernández, Marietta Tuena de Gómez-Puyou, Ruy Pérez-Montfort, Alfredo Torres-Larios, Armando Gómez Puyou
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3125179?pdf=render

Internet

http://europepmc.org/articles/PMC3125179?pdf=render

Similar Items