Extraction of high-quality RNA from mouse pancreatic tumors

Extraction of high-quality RNA from pancreatic tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in ribonucleases. The majority of the established RNA isolation protocols for use with primary pancreatic tissue involve perfusion of RNA stabilizing reagent int...

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Bibliographic Details
Main Authors: Kranthi Kumar Chougoni, Steven R. Grossman
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
RIN
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016120303836