Exopolysaccharide production by Lactobacillus confusus TISTR 1498 using coconut water as an alternative carbon source: the effect of peptone, yeast extract and beef extract

• Main Authors: Phisit Seesuriyachan, Ampin Kuntiya, Charin Techapun
• Format: Article
• Language: English
• Published: Prince of Songkla University 2011-08-01
• Series: Songklanakarin Journal of Science and Technology (SJST)
• Subjects: coconut water; fermentation; exopolysaccharide; batch culture; lactic acid bacteria; Lactobacillus confusus
• Online Access: http://rdo.psu.ac.th/sjstweb/journal/33-4/0125-3395-33-4-379-387.pdf

Coconut water (CW) is a by-product of food industry and has little value in Thailand. It is usually discarded as a wasteinto the environment. Consequently, we developed a value added process of exopolysaccharide (EPS) production usingLactobacillus confusus TISTR 1498 and coconut water. The effect of three expensive supplements (peptone, yeast extractand beef extract) on EPS and biomass production was investigated at 35°C for 24 h. Using a mod-MRS-CW medium, preparedby replacing the de-ionized water with 100% CW and supplemented with 20 g/l crystalline sucrose and a reduced quantity(50%) of the three expensive supplements (5 g/l of peptone, 2.5 g/l of yeast extract, and 2.5 g/l of beef extract) gave thehighest yield of EPS (12.3 g/l). By optimizing the conditions for fermentation (pH 5.5, agitation speed at 50 rpm and initialsucrose concentration of 100 g/l), EPS yield increased up to 38.2 g/l. When compared with the modified MRS medium, themedium supplemented with CW was found to be suitable for the reduction of cost spent on the organic nitrogen and growthfactors (savings close to 50%).