Identification and characterization of andalusicin: N-terminally dimethylated class III lantibiotic from Bacillus thuringiensis sv. andalousiensis

• Main Authors: Anastasiia Grigoreva, Julia Andreeva, Dmitry Bikmetov, Anastasiia Rusanova, Marina Serebryakova, Andrea Hernandez Garcia, Darya Slonova, Satish K. Nair, Guy Lippens, Konstantin Severinov, Svetlana Dubiley
• Format: Article
• Language: English
• Published: Elsevier 2021-05-01
• Series: iScience
• Subjects: Chemistry; Biosynthesis; Biochemistry; Medical biochemistry
• Online Access: http://www.sciencedirect.com/science/article/pii/S258900422100448X

Summary: Lanthipeptides, ribosomally synthesized and post-translationally modified peptides (RiPPs), can be divided into five classes based on their structures and biosynthetic pathways. Class I and II lanthipeptides have been well characterized, whereas less is known about members of the other three classes. Here, we describe a new family of class III lanthipeptides from Firmicutes. Members of the family are distinguished by the presence of a single carboxy-terminal labionin. We identified and characterized andalusicin, a representative of this family. Andalusicin bears two methyl groups at the α-amino terminus, a post-translational modification that has not previously been identified in class III lanthipeptides. Mature andalusicin A shows bioactivity against various Gram-positive bacteria, an activity that is highly dependent on the α-N dimethylation.