An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts

An Efficient and Economical Protocol for Isolating, Purifying and PEG-Mediated Transient Gene Expression of Chinese Kale Hypocotyl Protoplasts

In this study, we report the isolation and purification of protoplasts from Chinese kale (Brassica oleracea var. alboglabra) hypocotyls, and their transient gene expression transformation and subcellular localization of BaMYB75 (Bol042409). The upshot is that the vintage protocol included 5-d hypoco...

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Bibliographic Details
Main Authors: Bo Sun, Qiao Yuan, Hao Zheng, Sha Liang, Min Jiang, Mei-Mei Wang, Qing Chen, Meng-Yao Li, Yong Zhang, Ya Luo, Rong-Gao Gong, Fen Zhang, Hao-Ru Tang
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Plants
Subjects:
chinese kale
hypocotyl
transient expression
subcellular localization
bamyb75
Online Access:https://www.mdpi.com/2223-7747/8/10/385

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