Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy

Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy

Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.

Bibliographic Details
Main Authors: Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew
Format: Article
Language:English
Published: Nature Publishing Group 2020-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-20056-9
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spelling doaj-293de6b5849b489dbc388634cd7aeec12021-01-31T13:16:01ZengNature Publishing GroupNature Communications2041-17232020-12-0111111110.1038/s41467-020-20056-9Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopyHesam Mazidi0Tianben Ding1Arye Nehorai2Matthew D. Lew3Department of Electrical and Systems Engineering, Washington University in St. LouisDepartment of Electrical and Systems Engineering, Washington University in St. LouisDepartment of Electrical and Systems Engineering, Washington University in St. LouisDepartment of Electrical and Systems Engineering, Washington University in St. LouisStandard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.https://doi.org/10.1038/s41467-020-20056-9
collection DOAJ
language English
format Article
sources DOAJ
author Hesam Mazidi
Tianben Ding
Arye Nehorai
Matthew D. Lew
spellingShingle Hesam Mazidi
Tianben Ding
Arye Nehorai
Matthew D. Lew
Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
Nature Communications
author_facet Hesam Mazidi
Tianben Ding
Arye Nehorai
Matthew D. Lew
author_sort Hesam Mazidi
title Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
title_short Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
title_full Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
title_fullStr Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
title_full_unstemmed Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
title_sort quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2020-12-01
description Standard benchmarking of single-molecule localization microscopy cannot quantify nanoscale accuracy of arbitrary datasets. Here, the authors present Wasserstein-induced flux, a method using a chosen perturbation and knowledge of the imaging system to measure confidence of individual localizations.
url https://doi.org/10.1038/s41467-020-20056-9
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AT tianbending quantifyingaccuracyandheterogeneityinsinglemoleculesuperresolutionmicroscopy
AT aryenehorai quantifyingaccuracyandheterogeneityinsinglemoleculesuperresolutionmicroscopy
AT matthewdlew quantifyingaccuracyandheterogeneityinsinglemoleculesuperresolutionmicroscopy
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