Preliminary results of random amplification of polymorphic DNA among Triatominae of the phyllosoma complex (Hemiptera, Reduviidae)

• Main Authors: Brenière Simone F, Taveira Bruno, Bosseno, Ordoñez Rosalinda, Lozano-Kasten Felipe, Magallón-Gastélum Ezequiel, Ouaissi Ali, Ramsey Janine
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2003-01-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Triatominae; phyllosoma complex; random amplification of polymorphic DNA; phylogeny; Mexico
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000800010
• ISSN: 0074-0276; 1678-8060

In Mexico, Triatoma longipennis (Usinger), Triatoma picturata (Usinger), and Triatoma pallidipennis (Stal), primary Chagas disease vector species of the phyllosoma complex, were analyzed by randomly amplified polymorphic DNA (RAPD). Sixteen decametric primers resolved individual profiles not identical, but partially discriminative between species. Analysis based on pairwise presence/absence comparisons between the three species was performed using three primers and two outgroup species Triatoma infestans (Klug) and Triatoma barberi (Usinger). Fifty-three bands in total were scored, although only two bands were constant among the three phyllosoma complex species. Two other bands were constant only for T. longipennis and T. picturata together, and not present in T. pallidipennis. Neighbor Joining tree and the multiple correspondence analysis discriminated T. pallidipennis clearly from the other two species, although there was overlap between T. longipennis and T. picturata. The results indicate a close relationship between the studied species and support the hypothesis of their recent evolution. The suitability of RAPD to discern populations within the species is discussed.