Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats

Backgroud: Reactive oxygen species are known to play a major role in mercuric chloride induced oxidative and renal stress. Sodium selenite as an exogenous source of selenium is used for endogenous selenoprotein synthesis to scavenge the free radicals. The study was designed to investigate the possib...

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Main Authors: Necib, Youcef, Bahi, Ahlem, Zerizer, Sakina
Format: Article
Language:English
Published: "Vikol publishing" ST Kolesnichenko V.V. 2013-04-01
Series:Journal of Stress Physiology & Biochemistry
Subjects:
Online Access:http://www.jspb.ru/issues/2013/N2/JSPB_2013_2_159-172.pdf
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spelling doaj-28cfdd29c18a4b24afaf986495555af62020-11-24T22:50:18Zeng"Vikol publishing" ST Kolesnichenko V.V. Journal of Stress Physiology & Biochemistry1997-08382013-04-0192159172Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in RatsNecib, YoucefBahi, AhlemZerizer, SakinaBackgroud: Reactive oxygen species are known to play a major role in mercuric chloride induced oxidative and renal stress. Sodium selenite as an exogenous source of selenium is used for endogenous selenoprotein synthesis to scavenge the free radicals. The study was designed to investigate the possible protective role of sodium selenite in mercuric chloride induced renal stress, by using biochemical approaches. Adult male Albinos Wistar rats were randomly divided into four groups. The first group was served as the control, the second group was given sodium selenite (0.25 mg/kg b.w), while the third group was given mercuric chloride (0.25 mg/kg), finally, the fourth group was given combined treatment of sodium selenite and mercuric chloride for 3 weeks.Results: The effects of sodium selenite on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea, uric acid, billirubin levels and LDH activity, kidney tissue lipid peroxidation, GSH levels, GSH-Px, GST and catalase activities and hematological parameters. Administration of mercuric chloride induced significant increase in serum: creatinine, urea, uric acid and billirubin concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px, GST, and catalase activities along with increase the level of lipid peroxidation. Furthermore, treatment with mercuric chloride caused a marked elevation of kidney weight and decreased body weight and erythrocytes, hemoglobin, hematocrit levels. Sodium selenite treatment markedly reduced elevated serum: creatinine, urea, uric acid and billirubin levels, and LDH activity and conteracted the deterious effects of mercuric chloride on oxidative stress markers and hematological parameters and atteneuated histopathological changes caused by HgCl2 in kidney.Conclusion: Our results indicate that sodium selenite could have a beneficial role against mercuric chloride induced nephrotoxicity and oxidative stress in rat.http://www.jspb.ru/issues/2013/N2/JSPB_2013_2_159-172.pdfantioxidant enzymeslipid peroxidationmercuryrenal stresssodium selenite
collection DOAJ
language English
format Article
sources DOAJ
author Necib, Youcef
Bahi, Ahlem
Zerizer, Sakina
spellingShingle Necib, Youcef
Bahi, Ahlem
Zerizer, Sakina
Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats
Journal of Stress Physiology & Biochemistry
antioxidant enzymes
lipid peroxidation
mercury
renal stress
sodium selenite
author_facet Necib, Youcef
Bahi, Ahlem
Zerizer, Sakina
author_sort Necib, Youcef
title Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats
title_short Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats
title_full Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats
title_fullStr Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats
title_full_unstemmed Protective Role of Sodium Selenite on Mercuric Chloride Induced Oxidative and Renal Stress in Rats
title_sort protective role of sodium selenite on mercuric chloride induced oxidative and renal stress in rats
publisher "Vikol publishing" ST Kolesnichenko V.V.
series Journal of Stress Physiology & Biochemistry
issn 1997-0838
publishDate 2013-04-01
description Backgroud: Reactive oxygen species are known to play a major role in mercuric chloride induced oxidative and renal stress. Sodium selenite as an exogenous source of selenium is used for endogenous selenoprotein synthesis to scavenge the free radicals. The study was designed to investigate the possible protective role of sodium selenite in mercuric chloride induced renal stress, by using biochemical approaches. Adult male Albinos Wistar rats were randomly divided into four groups. The first group was served as the control, the second group was given sodium selenite (0.25 mg/kg b.w), while the third group was given mercuric chloride (0.25 mg/kg), finally, the fourth group was given combined treatment of sodium selenite and mercuric chloride for 3 weeks.Results: The effects of sodium selenite on mercuric chloride induced oxidative and renal stress were evaluated by serum creatinine, urea, uric acid, billirubin levels and LDH activity, kidney tissue lipid peroxidation, GSH levels, GSH-Px, GST and catalase activities and hematological parameters. Administration of mercuric chloride induced significant increase in serum: creatinine, urea, uric acid and billirubin concentration showing renal stress. Mercuric chloride also induced oxidative stress, as indicate by decreased kidney tissue of GSH level, GSH-Px, GST, and catalase activities along with increase the level of lipid peroxidation. Furthermore, treatment with mercuric chloride caused a marked elevation of kidney weight and decreased body weight and erythrocytes, hemoglobin, hematocrit levels. Sodium selenite treatment markedly reduced elevated serum: creatinine, urea, uric acid and billirubin levels, and LDH activity and conteracted the deterious effects of mercuric chloride on oxidative stress markers and hematological parameters and atteneuated histopathological changes caused by HgCl2 in kidney.Conclusion: Our results indicate that sodium selenite could have a beneficial role against mercuric chloride induced nephrotoxicity and oxidative stress in rat.
topic antioxidant enzymes
lipid peroxidation
mercury
renal stress
sodium selenite
url http://www.jspb.ru/issues/2013/N2/JSPB_2013_2_159-172.pdf
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