Development of a Novel Optical Biosensor for Detection of Organophoshorus Pesticides Based on Methyl Parathion Hydrolase Immobilized by Metal-Chelate Affinity

Development of a Novel Optical Biosensor for Detection of Organophoshorus Pesticides Based on Methyl Parathion Hydrolase Immobilized by Metal-Chelate Affinity

We have developed a novel optical biosensor device using recombinant methyl parathion hydrolase (MPH) enzyme immobilized on agarose by metal-chelate affinity to detect organophosphorus (OP) compounds with a nitrophenyl group. The biosensor principle is based on the optical measurement of the product...

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Bibliographic Details
Main Authors: Wensheng Lan, Guoping Chen, Feng Cui, Feng Tan, Ran Liu, Maolidan Yushupujiang
Format: Article
Language:English
Published: MDPI AG 2012-06-01
Series:Sensors
Subjects:
optical biosensor
methyl parathion hydrolase
organophosphorus compound
metal-chelate affinity
Online Access:http://www.mdpi.com/1424-8220/12/7/8477

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