Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3

Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3

In this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI was 1.1 fold when the stop codon was changed f...

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Main Authors: Zhang Xing, Fei Aiping, Wang Yong, Fang Zhigang, Teng Yingxue, Hao Xiaoliang
Format: Article
Language:English
Published: EDP Sciences 2018-01-01
Series:MATEC Web of Conferences
Online Access:https://doi.org/10.1051/matecconf/201823804003
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spelling doaj-28af973ee0f44f3e9cf21bb1c05182882021-02-02T04:25:35ZengEDP SciencesMATEC Web of Conferences2261-236X2018-01-012380400310.1051/matecconf/201823804003matecconf_icnfm2018_04003Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3Zhang XingFei AipingWang YongFang ZhigangTeng YingxueHao XiaoliangIn this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI was 1.1 fold when the stop codon was changed from UAA to UAG. For last sense codon TAG, the RFI of GFP(TAG) with the stop codon UAG was stronger than that with the stop codons UAA and UGA.https://doi.org/10.1051/matecconf/201823804003
collection DOAJ
language English
format Article
sources DOAJ
author Zhang Xing
Fei Aiping
Wang Yong
Fang Zhigang
Teng Yingxue
Hao Xiaoliang
spellingShingle Zhang Xing
Fei Aiping
Wang Yong
Fang Zhigang
Teng Yingxue
Hao Xiaoliang
Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
MATEC Web of Conferences
author_facet Zhang Xing
Fei Aiping
Wang Yong
Fang Zhigang
Teng Yingxue
Hao Xiaoliang
author_sort Zhang Xing
title Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
title_short Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
title_full Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
title_fullStr Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
title_full_unstemmed Influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in Escherichia coli when the expression vector pKK223-3
title_sort influence of the combination of last sense codon and stop codon on expression efficiency of green fluorescent protein gene in escherichia coli when the expression vector pkk223-3
publisher EDP Sciences
series MATEC Web of Conferences
issn 2261-236X
publishDate 2018-01-01
description In this paper, the influence of the combination of last sense codon and stop codon on expression efficiency was studied. By our study, for the last sense codon CCG, the RFI of GFP(CCG) was 2.1 fold when the stop codon was UAA, but in comparison, the RFI was 1.1 fold when the stop codon was changed from UAA to UAG. For last sense codon TAG, the RFI of GFP(TAG) with the stop codon UAG was stronger than that with the stop codons UAA and UGA.
url https://doi.org/10.1051/matecconf/201823804003
work_keys_str_mv AT zhangxing influenceofthecombinationoflastsensecodonandstopcodononexpressionefficiencyofgreenfluorescentproteingeneinescherichiacoliwhentheexpressionvectorpkk2233
AT feiaiping influenceofthecombinationoflastsensecodonandstopcodononexpressionefficiencyofgreenfluorescentproteingeneinescherichiacoliwhentheexpressionvectorpkk2233
AT wangyong influenceofthecombinationoflastsensecodonandstopcodononexpressionefficiencyofgreenfluorescentproteingeneinescherichiacoliwhentheexpressionvectorpkk2233
AT fangzhigang influenceofthecombinationoflastsensecodonandstopcodononexpressionefficiencyofgreenfluorescentproteingeneinescherichiacoliwhentheexpressionvectorpkk2233
AT tengyingxue influenceofthecombinationoflastsensecodonandstopcodononexpressionefficiencyofgreenfluorescentproteingeneinescherichiacoliwhentheexpressionvectorpkk2233
AT haoxiaoliang influenceofthecombinationoflastsensecodonandstopcodononexpressionefficiencyofgreenfluorescentproteingeneinescherichiacoliwhentheexpressionvectorpkk2233
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