Chromosome-Level Comprehensive Genome of Mangrove Sediment-Derived Fungus <i>Penicillium variabile</i> HXQ-H-1

• Main Authors: Ling Peng, Liangwei Li, Xiaochuan Liu, Jianwei Chen, Chengcheng Shi, Wenjie Guo, Qiwu Xu, Guangyi Fan, Xin Liu, Dehai Li
• Format: Article
• Language: English
• Published: MDPI AG 2019-12-01
• Series: Journal of Fungi
• Subjects: <i>penicillium variabile</i>; chromosome-level genome; mangrove; secondary metabolites; phylogeny
• Online Access: https://www.mdpi.com/2309-608X/6/1/7

<i>Penicillium</i> is an ascomycetous genus widely distributed in the natural environment and is one of the dominant fungi involved in the decomposition of mangroves, which can produce a variety of antitumor compounds and bioactive substances. However, in mangrove ecosystems there is no complete genome in this genus. In this study, we isolated a fungus strain named <i>Penicillium variabile</i> HXQ-H-1 from coast mangrove (Fujian Province, China). We generated a chromosome-level genome with total size of 33.32 Mb, scaffold N50 of 5.23 Mb and contig N50 of 96.74 kb. Additionally, we anchored about 95.91% assembly sequences into the longest seven scaffolds, and predicted 10,622 protein-coding genes, in which 99.66% could be annotated by eight protein databases. The secondary metabolites analysis reveals the strain has various gene clusters involving polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS) and terpene synthase that may have a largely capacity of biotechnological potential. Comparison genome analysis between <i>Penicillium variabile</i> and <i>Talaromyces islandicus</i> reveals a small difference in the total number of genes, whereas HXQ-H-1 has a higher gene number with COG functional annotation. Evolutionary relationship of <i>Penicillum</i> based on genome-wide data was carried out for the first time, showing the strain HXQ-H-1 is closely related to <i>Talaromyces islandicus</i>. This genomic resource may provide a new resource for development of novel bioactive antibiotics, drug candidates and precursors in <i>Penicillium variabile</i>.