A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies

Meloxicam is a non-steroidal anti-inflammatory drug, which has a preferential inhibitory effect to cyclooxyganase-2 (COX-2). Although the drug inhibits prostaglandin synthesis, the exact mechanism of meloxicam is still unknown. This is the first study to assess the effect of meloxicam on protein gly...

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Main Authors: Cezary Pawlukianiec, Małgorzata Ewa Gryciuk, Kacper Maksymilian Mil, Małgorzata Żendzian-Piotrowska, Anna Zalewska, Mateusz Maciejczyk
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Pharmaceuticals
Subjects:
Online Access:https://www.mdpi.com/1424-8247/13/9/240
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spelling doaj-28471446bc8849ec92d5f23b199e5c3c2020-11-25T03:44:27ZengMDPI AGPharmaceuticals1424-82472020-09-011324024010.3390/ph13090240A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro StudiesCezary Pawlukianiec0Małgorzata Ewa Gryciuk1Kacper Maksymilian Mil2Małgorzata Żendzian-Piotrowska3Anna Zalewska4Mateusz Maciejczyk5Students Scientific Club “Biochemistry of Civilization Diseases” at the Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandStudents Scientific Club “Biochemistry of Civilization Diseases” at the Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandStudents Scientific Club “Biochemistry of Civilization Diseases” at the Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandExperimental Dentistry Laboratory, Medical University of Bialystok, 24a M. Sklodowskiej-Curie Street, 15-274 Bialystok, PolandDepartment of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, 2c Mickiewicza Street, 15-233 Bialystok, PolandMeloxicam is a non-steroidal anti-inflammatory drug, which has a preferential inhibitory effect to cyclooxyganase-2 (COX-2). Although the drug inhibits prostaglandin synthesis, the exact mechanism of meloxicam is still unknown. This is the first study to assess the effect of meloxicam on protein glyco-oxidation as well as antioxidant activity. For this purpose, we used an in vitro model of oxidized bovine serum albumin (BSA). Glucose, fructose, ribose, glyoxal and methylglyoxal were used as glycating agents, while chloramine T was used as an oxidant. We evaluated the antioxidant properties of albumin (2,2-di-phenyl-1-picrylhydrazyl radical scavenging capacity, total antioxidant capacity and ferric reducing antioxidant power), the intensity of protein glycation (Amadori products, advanced glycation end products) and glyco-oxidation (dityrosine, kynurenine, N-formylkynurenine, tryptophan and amyloid-β) as well as the content of protein oxidation products (advanced oxidation protein products, carbonyl groups and thiol groups). We have demonstrated that meloxicam enhances the antioxidant properties of albumin and prevents the protein oxidation and glycation under the influence of various factors such as sugars, aldehydes and oxidants. Importantly, the antioxidant and anti-glycating activity is similar to that of routinely used antioxidants such as captopril, Trolox, reduced glutathione and lipoic acid as well as protein glycation inhibitors (aminoguanidine). Pleiotropic action of meloxicam may increase the effectiveness of anti-inflammatory treatment in diseases with oxidative stress etiology.https://www.mdpi.com/1424-8247/13/9/240meloxicamprotein glycationprotein oxidationprotein glyco-oxidationantioxidant activity
collection DOAJ
language English
format Article
sources DOAJ
author Cezary Pawlukianiec
Małgorzata Ewa Gryciuk
Kacper Maksymilian Mil
Małgorzata Żendzian-Piotrowska
Anna Zalewska
Mateusz Maciejczyk
spellingShingle Cezary Pawlukianiec
Małgorzata Ewa Gryciuk
Kacper Maksymilian Mil
Małgorzata Żendzian-Piotrowska
Anna Zalewska
Mateusz Maciejczyk
A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies
Pharmaceuticals
meloxicam
protein glycation
protein oxidation
protein glyco-oxidation
antioxidant activity
author_facet Cezary Pawlukianiec
Małgorzata Ewa Gryciuk
Kacper Maksymilian Mil
Małgorzata Żendzian-Piotrowska
Anna Zalewska
Mateusz Maciejczyk
author_sort Cezary Pawlukianiec
title A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies
title_short A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies
title_full A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies
title_fullStr A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies
title_full_unstemmed A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro Studies
title_sort new insight into meloxicam: assessment of antioxidant and anti-glycating activity in in vitro studies
publisher MDPI AG
series Pharmaceuticals
issn 1424-8247
publishDate 2020-09-01
description Meloxicam is a non-steroidal anti-inflammatory drug, which has a preferential inhibitory effect to cyclooxyganase-2 (COX-2). Although the drug inhibits prostaglandin synthesis, the exact mechanism of meloxicam is still unknown. This is the first study to assess the effect of meloxicam on protein glyco-oxidation as well as antioxidant activity. For this purpose, we used an in vitro model of oxidized bovine serum albumin (BSA). Glucose, fructose, ribose, glyoxal and methylglyoxal were used as glycating agents, while chloramine T was used as an oxidant. We evaluated the antioxidant properties of albumin (2,2-di-phenyl-1-picrylhydrazyl radical scavenging capacity, total antioxidant capacity and ferric reducing antioxidant power), the intensity of protein glycation (Amadori products, advanced glycation end products) and glyco-oxidation (dityrosine, kynurenine, N-formylkynurenine, tryptophan and amyloid-β) as well as the content of protein oxidation products (advanced oxidation protein products, carbonyl groups and thiol groups). We have demonstrated that meloxicam enhances the antioxidant properties of albumin and prevents the protein oxidation and glycation under the influence of various factors such as sugars, aldehydes and oxidants. Importantly, the antioxidant and anti-glycating activity is similar to that of routinely used antioxidants such as captopril, Trolox, reduced glutathione and lipoic acid as well as protein glycation inhibitors (aminoguanidine). Pleiotropic action of meloxicam may increase the effectiveness of anti-inflammatory treatment in diseases with oxidative stress etiology.
topic meloxicam
protein glycation
protein oxidation
protein glyco-oxidation
antioxidant activity
url https://www.mdpi.com/1424-8247/13/9/240
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