Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
Aim: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. Materials and Methods: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were ino...
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doaj-283298f6857d43e09315d78b5e1fc7212021-08-02T08:06:36ZengVeterinary WorldVeterinary World0972-89882231-09162017-05-0110551752010.14202/vetworld.2017.517-520Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West BengalArnab Das0Chanchal Guha1Ujjwal Biswas2Partha Sarathi Jana3Amaresh Chatterjee4Indranil Samanta5Animal Resources Development Department, Institute of Animal Health and Veterinary Biologicals, Government of West Bengal, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Aim: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. Materials and Methods: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of blaCTX-M, blaTEM, blaSHV, blaVIM, tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance. Results: In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, blaCTX-M was detected in 18 (36%) isolates, and 6 (12%) harbored blaTEM genes in PCR. None of the isolates carried blaSHV genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates. Conclusion: This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India.http://www.veterinaryworld.org/Vol.10/May-2017/8.pdfantibiotic resistancecattleEscherichia coliIndiasub-clinical mastitis |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arnab Das Chanchal Guha Ujjwal Biswas Partha Sarathi Jana Amaresh Chatterjee Indranil Samanta |
spellingShingle |
Arnab Das Chanchal Guha Ujjwal Biswas Partha Sarathi Jana Amaresh Chatterjee Indranil Samanta Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal Veterinary World antibiotic resistance cattle Escherichia coli India sub-clinical mastitis |
author_facet |
Arnab Das Chanchal Guha Ujjwal Biswas Partha Sarathi Jana Amaresh Chatterjee Indranil Samanta |
author_sort |
Arnab Das |
title |
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal |
title_short |
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal |
title_full |
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal |
title_fullStr |
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal |
title_full_unstemmed |
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal |
title_sort |
detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in west bengal |
publisher |
Veterinary World |
series |
Veterinary World |
issn |
0972-8988 2231-0916 |
publishDate |
2017-05-01 |
description |
Aim: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal.
Materials and Methods: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of blaCTX-M, blaTEM, blaSHV, blaVIM, tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance.
Results: In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, blaCTX-M was detected in 18 (36%) isolates, and 6 (12%) harbored blaTEM genes in PCR. None of the isolates carried blaSHV genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates.
Conclusion: This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India. |
topic |
antibiotic resistance cattle Escherichia coli India sub-clinical mastitis |
url |
http://www.veterinaryworld.org/Vol.10/May-2017/8.pdf |
work_keys_str_mv |
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