Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal

Aim: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. Materials and Methods: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were ino...

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Main Authors: Arnab Das, Chanchal Guha, Ujjwal Biswas, Partha Sarathi Jana, Amaresh Chatterjee, Indranil Samanta
Format: Article
Language:English
Published: Veterinary World 2017-05-01
Series:Veterinary World
Subjects:
Online Access:http://www.veterinaryworld.org/Vol.10/May-2017/8.pdf
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spelling doaj-283298f6857d43e09315d78b5e1fc7212021-08-02T08:06:36ZengVeterinary WorldVeterinary World0972-89882231-09162017-05-0110551752010.14202/vetworld.2017.517-520Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West BengalArnab Das0Chanchal Guha1Ujjwal Biswas2Partha Sarathi Jana3Amaresh Chatterjee4Indranil Samanta5Animal Resources Development Department, Institute of Animal Health and Veterinary Biologicals, Government of West Bengal, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Epidemiology and Preventive Medicine, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, West Bengal, India.Aim: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. Materials and Methods: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of blaCTX-M, blaTEM, blaSHV, blaVIM, tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance. Results: In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, blaCTX-M was detected in 18 (36%) isolates, and 6 (12%) harbored blaTEM genes in PCR. None of the isolates carried blaSHV genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates. Conclusion: This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India.http://www.veterinaryworld.org/Vol.10/May-2017/8.pdfantibiotic resistancecattleEscherichia coliIndiasub-clinical mastitis
collection DOAJ
language English
format Article
sources DOAJ
author Arnab Das
Chanchal Guha
Ujjwal Biswas
Partha Sarathi Jana
Amaresh Chatterjee
Indranil Samanta
spellingShingle Arnab Das
Chanchal Guha
Ujjwal Biswas
Partha Sarathi Jana
Amaresh Chatterjee
Indranil Samanta
Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
Veterinary World
antibiotic resistance
cattle
Escherichia coli
India
sub-clinical mastitis
author_facet Arnab Das
Chanchal Guha
Ujjwal Biswas
Partha Sarathi Jana
Amaresh Chatterjee
Indranil Samanta
author_sort Arnab Das
title Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_short Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_full Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_fullStr Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_full_unstemmed Detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in West Bengal
title_sort detection of emerging antibiotic resistance in bacteria isolated from subclinical mastitis in cattle in west bengal
publisher Veterinary World
series Veterinary World
issn 0972-8988
2231-0916
publishDate 2017-05-01
description Aim: The aim of this work was to detect antibiotic resistance in Gram-negative bacteria isolated from subclinical mastitis in cattle in West Bengal. Materials and Methods: The milk samples were collected from the cattle suffering with subclinical mastitis in West Bengal. The milk samples were inoculated into the nutrient broth and incubated at 37°C. On the next day, the growth was transferred into nutrient agar and MacConkey agar. All the pure cultures obtained from nutrient agar slant were subjected to Gram-staining and standard biochemical tests. All the bacterial isolates were tested in vitro for their sensitivity to different antibiotics commonly used in veterinary practices. All Gram-negative isolates including positive control were subjected to polymerase chain reaction (PCR) for detection of blaCTX-M, blaTEM, blaSHV, blaVIM, tetA, tetB, tetC, and tetM genes considered for extended-spectrum β-lactamase (ESBL), metallo-β-lactamase, and tetracycline resistance. Results: In total, 50 Gram-negative organisms (Escherichia coli, Proteus, Pseudomonas, Klebsiella, and Enterobacter) were isolated from milk samples of subclinical mastitis infected cattle. Among these Gram-negative isolates, 48% (24/50) were found either ESBL producing or tetracycline resistant. Out of total 50 Gram-negative isolates, blaCTX-M was detected in 18 (36%) isolates, and 6 (12%) harbored blaTEM genes in PCR. None of the isolates carried blaSHV genes. Further, in this study, 5 (10%) isolates harbored tet(A) gene, and 8 (16%) isolates carried tet(B) gene. No tet(C) gene was detected from the isolates. Conclusion: This study showed emerging trend of antibiotic-resistant Gram-negative bacteria associated with subclinical mastitis in cattle in West Bengal, India.
topic antibiotic resistance
cattle
Escherichia coli
India
sub-clinical mastitis
url http://www.veterinaryworld.org/Vol.10/May-2017/8.pdf
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