Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing

Codon-Precise, Synthetic, Antibody Fragment Libraries Built Using Automated Hexamer Codon Additions and Validated through Next Generation Sequencing

We have previously described ProxiMAX, a technology that enables the fabrication of precise, combinatorial gene libraries via codon-by-codon saturation mutagenesis. ProxiMAX was originally performed using manual, enzymatic transfer of codons via blunt-end ligation. Here we present Colibra™: an autom...

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Bibliographic Details
Main Authors: Laura Frigotto, Matthew E. Smith, Christopher Brankin, Ashni Sedani, Simon E. Cooper, Nisha Kanwar, Daniel Evans, Stanislava Svobodova, Claudia Baar, Jacob Glanville, Christopher G. Ullman, Anna V. Hine
Format: Article
Language:English
Published: MDPI AG 2015-05-01
Series:Antibodies
Subjects:
Colibra
ProxiMAX randomization
saturation mutagenesis
CDR
single domain antibody
camelid antibody
antibody engineering
Online Access:http://www.mdpi.com/2073-4468/4/2/88

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