Expression of Ang-2 and VEGF in the endometriosis

• Main Authors: Xiao-Li Chen, Gui-Qing Jiao
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-01-01
• Series: Journal of Hainan Medical University
• Subjects: Ang-2; VEGF; EMT; The Fifth Hospital of Zhangjiakou City in Hebei Province; 075000. Tel: [phone] E-mail: [e-mail] Foundation Project: The study was supported by the Scientific and Technological Research and Development Guiding Planning Project of Zhangjiakou City with the number of 0921122D. 1.; Expression
• Online Access: http://www.hnykdxxb.com/PDF/201602/19.pdf

Objective: To detect the expression of Ang-2 and VEGF in the endometriosis (EMT), and to explore the correlation of Ang-2 and VEGF with the pathogenesis of EMT. Methods: A total of 60 patients with EMT who were confirmed by laparoscopy and admitted in our hospital from August, 2014 to August, 2015 were included in the study and served as the observation group; moreover, 60 women who came for laparoscopy at the same stage were served as the control group. A volume of 5mL morning fasting elbow venous blood in the two groups was extracted, standing in the room temperature for 1h, centrifuged at 3 500 r/min for 20 min. The supernatant was extracted and stored at -70 ℃ for inspection. During the laparoscopy, a volume of 5 mL peritoneal fluid was collected, standing in the room temperature for 1 h, centrifuged at 3 500 r/min for 20 min. The supernatant was extracted and stored at -70 ℃ for inspection. ELISA was used to detect the levels of Ang-2 and VEGF. Results: The levels of Ang-2 and VEGF in the serum and peritoneal fluid in the observation group were significantly higher than those in the control group (P<0.05). The serum Ang-2 level in the severe patients was significantly higher than that in the mild patients, while the comparison of VEGF was not statistically significant. The levels of Ang-2 and VEGF in the peritoneal fluid in the severe patients were significantly higher than those in the mild patients. Conclusions: Ang-2 and VEGF can promote the angiogenesis in EMT patients, and their high expressions in the serum and peritoneal fluid are correlated with the occurrence and development of EMT; therefore, detection of the levels of Ang-2 and VEGF is of great significance in estimating the progression of EMT in the clinic.