Synthetic Promoters and Transcription Factors for Heterologous Protein Expression in Saccharomyces cerevisiae

Synthetic Promoters and Transcription Factors for Heterologous Protein Expression in Saccharomyces cerevisiae

Orthogonal systems for heterologous protein expression as well as for the engineering of synthetic gene regulatory circuits in hosts like Saccharomyces cerevisiae depend on synthetic transcription factors (synTFs) and corresponding cis-regulatory binding sites. We have constructed and characterized...

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Bibliographic Details
Main Authors: Fabian Machens, Salma Balazadeh, Bernd Mueller-Roeber, Katrin Messerschmidt
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-10-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
JUB1
synthetic biology
transcriptional regulation
gene expression
synthetic circuits
dead Cas9
Online Access:http://journal.frontiersin.org/article/10.3389/fbioe.2017.00063/full

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