Cloning and expression of gene encoding P23 protein from Cryptosporidium parvum

Cloning and expression of gene encoding P23 protein from Cryptosporidium parvum

We cloned the cp23 gene coding P23 (glyco)protein from Cryptosporidium parvum isolated from Thua Thien Hue province, Vietnam. The coding region of cp23 gene from C. parvum is 99% similar with cp23 gene deposited in NCBI (accession number: U34390). SDS-PAGE and Western blot analysis showed that the c...

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Bibliographic Details
Main Authors: Dinh Thi Bich Lan, Tran Thuy Lan, Le Quoc Viet, Phan Van Quyet, Hoang Tan Quang, Nguyen Hoang Loc, Phung Thang Long
Format: Article
Language:English
Published: Plovdiv University Press 2014-12-01
Series:Journal of BioScience and Biotechnology
Subjects:
cp23 gene
Cryptosporidium parvum
P23 protein
Online Access:http://www.jbb.uni-plovdiv.bg/documents/27807/352488/jbb_2014-3(3)-pages_189-193.pdf
Description
Summary:We cloned the cp23 gene coding P23 (glyco)protein from Cryptosporidium parvum isolated from Thua Thien Hue province, Vietnam. The coding region of cp23 gene from C. parvum is 99% similar with cp23 gene deposited in NCBI (accession number: U34390). SDS-PAGE and Western blot analysis showed that the cp23 gene in E. coli BL21 StarTM (DE3) produced polypeptides with molecular weights of approximately 37, 40 and 49 kDa. These molecules may be non-glycosylated or glycosylated P23 fusion polypeptides. Recombinant P23 protein purified by GST (glutathione S-transferase) affinity chromatography can be used as an antigen for C. parvum antibody production as well as to develop diagnostic kit for C. parvum.
ISSN:1314-6238
1314-6246

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