High-efficiency RNA-based reprogramming of human primary fibroblasts

Induced pluripotent stem cells (iPSCs) have potential for regenerative medicine applications, but are generated with very low efficiency. Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA...

Full description

Bibliographic Details
Main Authors:	Igor Kogut, Sandra M. McCarthy, Maryna Pavlova, David P. Astling, Xiaomi Chen, Ana Jakimenko, Kenneth L. Jones, Andrew Getahun, John C. Cambier, Anna M. G. Pasmooij, Marcel F. Jonkman, Dennis R. Roop, Ganna Bilousova
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-02-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-03190-3

id	doaj-279523f467794ff7bb0f3d426b9968a3
record_format	Article
spelling	doaj-279523f467794ff7bb0f3d426b9968a32021-05-11T09:28:41ZengNature Publishing GroupNature Communications2041-17232018-02-019111510.1038/s41467-018-03190-3High-efficiency RNA-based reprogramming of human primary fibroblastsIgor Kogut0Sandra M. McCarthy1Maryna Pavlova2David P. Astling3Xiaomi Chen4Ana Jakimenko5Kenneth L. Jones6Andrew Getahun7John C. Cambier8Anna M. G. Pasmooij9Marcel F. Jonkman10Dennis R. Roop11Ganna Bilousova12Department of Dermatology, University of Colorado School of MedicineDepartment of Dermatology, University of Colorado School of MedicineDepartment of Dermatology, University of Colorado School of MedicineDepartment of Biochemistry and Molecular Genetics, University of Colorado School of MedicineDepartment of Dermatology, University of Colorado School of MedicineDepartment of Dermatology, University of Colorado School of MedicineDepartment of Pediatrics, University of Colorado School of MedicineDepartment of Immunology and Microbiology, University of Colorado School of MedicineDepartment of Immunology and Microbiology, University of Colorado School of MedicineDepartment of Dermatology, University Medical CenterDepartment of Dermatology, University Medical CenterDepartment of Dermatology, University of Colorado School of MedicineDepartment of Dermatology, University of Colorado School of MedicineInduced pluripotent stem cells (iPSCs) have potential for regenerative medicine applications, but are generated with very low efficiency. Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA mimics, and optimized culture methods.https://doi.org/10.1038/s41467-018-03190-3
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Igor Kogut Sandra M. McCarthy Maryna Pavlova David P. Astling Xiaomi Chen Ana Jakimenko Kenneth L. Jones Andrew Getahun John C. Cambier Anna M. G. Pasmooij Marcel F. Jonkman Dennis R. Roop Ganna Bilousova
spellingShingle	Igor Kogut Sandra M. McCarthy Maryna Pavlova David P. Astling Xiaomi Chen Ana Jakimenko Kenneth L. Jones Andrew Getahun John C. Cambier Anna M. G. Pasmooij Marcel F. Jonkman Dennis R. Roop Ganna Bilousova High-efficiency RNA-based reprogramming of human primary fibroblasts Nature Communications
author_facet	Igor Kogut Sandra M. McCarthy Maryna Pavlova David P. Astling Xiaomi Chen Ana Jakimenko Kenneth L. Jones Andrew Getahun John C. Cambier Anna M. G. Pasmooij Marcel F. Jonkman Dennis R. Roop Ganna Bilousova
author_sort	Igor Kogut
title	High-efficiency RNA-based reprogramming of human primary fibroblasts
title_short	High-efficiency RNA-based reprogramming of human primary fibroblasts
title_full	High-efficiency RNA-based reprogramming of human primary fibroblasts
title_fullStr	High-efficiency RNA-based reprogramming of human primary fibroblasts
title_full_unstemmed	High-efficiency RNA-based reprogramming of human primary fibroblasts
title_sort	high-efficiency rna-based reprogramming of human primary fibroblasts
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-02-01
description	Induced pluripotent stem cells (iPSCs) have potential for regenerative medicine applications, but are generated with very low efficiency. Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA mimics, and optimized culture methods.
url	https://doi.org/10.1038/s41467-018-03190-3
work_keys_str_mv	AT igorkogut highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT sandrammccarthy highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT marynapavlova highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT davidpastling highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT xiaomichen highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT anajakimenko highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT kennethljones highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT andrewgetahun highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT johnccambier highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT annamgpasmooij highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT marcelfjonkman highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT dennisrroop highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts AT gannabilousova highefficiencyrnabasedreprogrammingofhumanprimaryfibroblasts
_version_	1721449733305663488

High-efficiency RNA-based reprogramming of human primary fibroblasts

Similar Items