Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study

Abstract Background Considered as one of the major causes of low back pain, Intervertebral disc degeneration (IVDD) is caused by several genetic and environmental factors. As inflammation plays an important role in disc degeneration, the genetic changes in both inflammatory and anti-inflammatory gen...

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Main Authors: Sara Hanaei, Sina Abdollahzade, Maryam Sadr, Mohammad Hossein Mirbolouk, Alireza Khoshnevisan, Nima Rezaei
Format: Article
Language:English
Published: BMC 2018-04-01
Series:BMC Medical Genetics
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12881-018-0572-2
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spelling doaj-278538b589d84dbd8358152cee6cd98d2021-04-02T10:21:22ZengBMCBMC Medical Genetics1471-23502018-04-0119111010.1186/s12881-018-0572-2Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control studySara Hanaei0Sina Abdollahzade1Maryam Sadr2Mohammad Hossein Mirbolouk3Alireza Khoshnevisan4Nima Rezaei5Molecular Immunology Research Center, Tehran University of Medical SciencesDepartment of Neurosurgery, Shariati Hospital, Tehran University of Medical SciencesMolecular Immunology Research Center, Tehran University of Medical SciencesDepartment of Neurosurgery, Shariati Hospital, Tehran University of Medical SciencesDepartment of Neurosurgery, Shariati Hospital, Tehran University of Medical SciencesResearch Center for Immunodeficiencies, Children’s Medical Center, Tehran University of Medical SciencesAbstract Background Considered as one of the major causes of low back pain, Intervertebral disc degeneration (IVDD) is caused by several genetic and environmental factors. As inflammation plays an important role in disc degeneration, the genetic changes in both inflammatory and anti-inflammatory genes may play causative roles in IVDD as well. Therefore, the interactions between inflammatory and anti-inflammatory cytokines and also other components of disc matrix would determine the degree of tissue destruction in disc degeneration. However, there is still controversy regarding the exact role of inflammation and disc homeostasis imbalance in pathophysiology of IVDD. Therefore, current study was conducted to investigate the role of IL-10 and TGF-β single nucleotide polymorphisms (SNP) in Iranian IVDD patients. Methods Seventy-six IVDD patients and 140 healthy controls were enrolled in this study. Genomic DNA from peripheral leukocytes was tested for 3 SNPs in IL10 (L-10 -1082G/A (rs1800896), IL-10 -819C/T (rs1800871), IL-10 -592A/C (rs1800872)) and 2 SNPs in TGF-β (TGF-β Codon 10 C/T (rs1982037), and TGF-β Codon 25 C/T (rs1800471) genes through PCR-SSP method. The extracted genomic DNA was genotyped for the aforementioned SNPs of interest using specific primers, which were coated in the cytokines KITs and based on the PCR-SSP method for sequencing. Results The ‘T’ allele of IL-10 -819C/T and the ‘C’ allele of IL-10 -592A/C were more prevalent among patients, whereas the ‘C’ and ‘A’ alleles of respective SNPs were significantly more frequent in controls. The genotypes including ‘CT’ of IL-10 -819C/T, ‘CA’ of IL-10 -592A/C, and ‘GA’ of IL-10 -1082A/G were more common among patients, while the ‘CC’ genotype of both IL-10 -819C/T and IL-10 -592A/C SNPs were more frequent in controls. In addition, the IL-10 haplotypes including ‘ACC’, ‘ATA’, and ‘ACA’ were significantly associated with disease. Meanwhile, the ‘TC’ haplotype of TGF-β was more common among patients as well. Conclusions The IL-10 SNPs were significantly associated with IVDD in Iranian population; which proposes that genomic alterations of anti-inflammatory cytokines could lead to homeostasis imbalance in intervertebral discs and degenerative changes.http://link.springer.com/article/10.1186/s12881-018-0572-2Intervertebral disc degenerationSingle nucleotide polymorphismInterleukin 10Transforming growth factor β
collection DOAJ
language English
format Article
sources DOAJ
author Sara Hanaei
Sina Abdollahzade
Maryam Sadr
Mohammad Hossein Mirbolouk
Alireza Khoshnevisan
Nima Rezaei
spellingShingle Sara Hanaei
Sina Abdollahzade
Maryam Sadr
Mohammad Hossein Mirbolouk
Alireza Khoshnevisan
Nima Rezaei
Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study
BMC Medical Genetics
Intervertebral disc degeneration
Single nucleotide polymorphism
Interleukin 10
Transforming growth factor β
author_facet Sara Hanaei
Sina Abdollahzade
Maryam Sadr
Mohammad Hossein Mirbolouk
Alireza Khoshnevisan
Nima Rezaei
author_sort Sara Hanaei
title Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study
title_short Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study
title_full Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study
title_fullStr Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study
title_full_unstemmed Association of IL10 and TGFB single nucleotide polymorphisms with intervertebral disc degeneration in Iranian population: a case control study
title_sort association of il10 and tgfb single nucleotide polymorphisms with intervertebral disc degeneration in iranian population: a case control study
publisher BMC
series BMC Medical Genetics
issn 1471-2350
publishDate 2018-04-01
description Abstract Background Considered as one of the major causes of low back pain, Intervertebral disc degeneration (IVDD) is caused by several genetic and environmental factors. As inflammation plays an important role in disc degeneration, the genetic changes in both inflammatory and anti-inflammatory genes may play causative roles in IVDD as well. Therefore, the interactions between inflammatory and anti-inflammatory cytokines and also other components of disc matrix would determine the degree of tissue destruction in disc degeneration. However, there is still controversy regarding the exact role of inflammation and disc homeostasis imbalance in pathophysiology of IVDD. Therefore, current study was conducted to investigate the role of IL-10 and TGF-β single nucleotide polymorphisms (SNP) in Iranian IVDD patients. Methods Seventy-six IVDD patients and 140 healthy controls were enrolled in this study. Genomic DNA from peripheral leukocytes was tested for 3 SNPs in IL10 (L-10 -1082G/A (rs1800896), IL-10 -819C/T (rs1800871), IL-10 -592A/C (rs1800872)) and 2 SNPs in TGF-β (TGF-β Codon 10 C/T (rs1982037), and TGF-β Codon 25 C/T (rs1800471) genes through PCR-SSP method. The extracted genomic DNA was genotyped for the aforementioned SNPs of interest using specific primers, which were coated in the cytokines KITs and based on the PCR-SSP method for sequencing. Results The ‘T’ allele of IL-10 -819C/T and the ‘C’ allele of IL-10 -592A/C were more prevalent among patients, whereas the ‘C’ and ‘A’ alleles of respective SNPs were significantly more frequent in controls. The genotypes including ‘CT’ of IL-10 -819C/T, ‘CA’ of IL-10 -592A/C, and ‘GA’ of IL-10 -1082A/G were more common among patients, while the ‘CC’ genotype of both IL-10 -819C/T and IL-10 -592A/C SNPs were more frequent in controls. In addition, the IL-10 haplotypes including ‘ACC’, ‘ATA’, and ‘ACA’ were significantly associated with disease. Meanwhile, the ‘TC’ haplotype of TGF-β was more common among patients as well. Conclusions The IL-10 SNPs were significantly associated with IVDD in Iranian population; which proposes that genomic alterations of anti-inflammatory cytokines could lead to homeostasis imbalance in intervertebral discs and degenerative changes.
topic Intervertebral disc degeneration
Single nucleotide polymorphism
Interleukin 10
Transforming growth factor β
url http://link.springer.com/article/10.1186/s12881-018-0572-2
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