ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development

ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development

In maize, shoot-borne roots dominate the whole root system and play essential roles in water and nutrient acquisition and lodging tolerance. Shoot-borne roots initiate at shoot nodes, including crown roots from the belowground nodes and brace roots from aboveground nodes. In contrast to crown roots,...

Full description

Bibliographic Details
Main Authors: Jieping Li, Fanjun Chen, Yanqing Li, Pengcheng Li, Yuanqing Wang, Guohua Mi, Lixing Yuan
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-07-01
Series:Frontiers in Plant Science
Subjects:
AP2 transcription factor
brace roots
candidate gene association
shoot-borne roots
root development
Zea mays L.
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2019.00820/full
id doaj-276e3d2a67ba4546ba658eeca27ea97a
record_format Article
spelling doaj-276e3d2a67ba4546ba658eeca27ea97a2020-11-24T23:55:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-07-011010.3389/fpls.2019.00820453067ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots DevelopmentJieping Li0Jieping Li1Fanjun Chen2Yanqing Li3Pengcheng Li4Pengcheng Li5Yuanqing Wang6Guohua Mi7Lixing Yuan8Key Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of Plant Science, School of Life Sciences, Henan University, Kaifeng, ChinaKey Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaJiangsu Key Laboratory of Crop Genetics and Physiology, Key Laboratory of Plant Functional Genomics, Co-Innovation Center for Modern Production Technology of Grain Crops, MOE, College of Agriculture, Yangzhou University, Yangzhou, ChinaKey Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaKey Laboratory of Plant-Soil Interaction, MOE, Department of Plant Nutrition, College Resources and Environmental Sciences, China Agricultural University, Beijing, ChinaIn maize, shoot-borne roots dominate the whole root system and play essential roles in water and nutrient acquisition and lodging tolerance. Shoot-borne roots initiate at shoot nodes, including crown roots from the belowground nodes and brace roots from aboveground nodes. In contrast to crown roots, few genes for brace roots development have been identified. Here, we characterized a maize AP2/ERF transcription factor, ZmRAP2.7, to be involved in brace roots development. ZmRAP2.7 expressed in all types of roots, and the encoded protein localized in the nucleus with transcriptional activation activity. A maize transposon insert mutant RAP2.7-Mu defective in ZmRAP2.7 expression revealed a decreased number of brace roots but not crown roots. Maize Corngrass1 mutant, which showed an elevated expression of ZmRAP2.7, however, revealed an increased number of brace roots. The ZmRAP2.7-based association analysis in a maize panel further identified a SNP marker at the fifth exon of gene to be associated with number of brace roots. These results uncovered a function of ZmRAP2.7 in brace roots development and provided the valuable gene and allele for genetic improvement of maize root systems.https://www.frontiersin.org/article/10.3389/fpls.2019.00820/fullAP2 transcription factorbrace rootscandidate gene associationshoot-borne rootsroot developmentZea mays L.
collection DOAJ
language English
format Article
sources DOAJ
author Jieping Li
Jieping Li
Fanjun Chen
Yanqing Li
Pengcheng Li
Pengcheng Li
Yuanqing Wang
Guohua Mi
Lixing Yuan
spellingShingle Jieping Li
Jieping Li
Fanjun Chen
Yanqing Li
Pengcheng Li
Pengcheng Li
Yuanqing Wang
Guohua Mi
Lixing Yuan
ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development
Frontiers in Plant Science
AP2 transcription factor
brace roots
candidate gene association
shoot-borne roots
root development
Zea mays L.
author_facet Jieping Li
Jieping Li
Fanjun Chen
Yanqing Li
Pengcheng Li
Pengcheng Li
Yuanqing Wang
Guohua Mi
Lixing Yuan
author_sort Jieping Li
title ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development
title_short ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development
title_full ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development
title_fullStr ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development
title_full_unstemmed ZmRAP2.7, an AP2 Transcription Factor, Is Involved in Maize Brace Roots Development
title_sort zmrap2.7, an ap2 transcription factor, is involved in maize brace roots development
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2019-07-01
description In maize, shoot-borne roots dominate the whole root system and play essential roles in water and nutrient acquisition and lodging tolerance. Shoot-borne roots initiate at shoot nodes, including crown roots from the belowground nodes and brace roots from aboveground nodes. In contrast to crown roots, few genes for brace roots development have been identified. Here, we characterized a maize AP2/ERF transcription factor, ZmRAP2.7, to be involved in brace roots development. ZmRAP2.7 expressed in all types of roots, and the encoded protein localized in the nucleus with transcriptional activation activity. A maize transposon insert mutant RAP2.7-Mu defective in ZmRAP2.7 expression revealed a decreased number of brace roots but not crown roots. Maize Corngrass1 mutant, which showed an elevated expression of ZmRAP2.7, however, revealed an increased number of brace roots. The ZmRAP2.7-based association analysis in a maize panel further identified a SNP marker at the fifth exon of gene to be associated with number of brace roots. These results uncovered a function of ZmRAP2.7 in brace roots development and provided the valuable gene and allele for genetic improvement of maize root systems.
topic AP2 transcription factor
brace roots
candidate gene association
shoot-borne roots
root development
Zea mays L.
url https://www.frontiersin.org/article/10.3389/fpls.2019.00820/full
work_keys_str_mv AT jiepingli zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT jiepingli zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT fanjunchen zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT yanqingli zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT pengchengli zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT pengchengli zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT yuanqingwang zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT guohuami zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
AT lixingyuan zmrap27anap2transcriptionfactorisinvolvedinmaizebracerootsdevelopment
_version_ 1725461483068850176

Similar Items