Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain

This study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided...

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Main Authors: Qian Wang, Jing Lin, Peng Yang, Yingye Liang, Dongming Lu, Kailong Wang, Wei Gan, Jianping Fu, Zhenbao Gan, Mingchen Ma, Pingting Wu, Fengshi He, Jun Pang, Hongliang Tang
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:Pain Research and Management
Online Access:http://dx.doi.org/10.1155/2020/8309745
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spelling doaj-2761a8d92dd34adf80300eb21bcf0bc82020-12-28T01:30:42ZengHindawi LimitedPain Research and Management1918-15232020-01-01202010.1155/2020/8309745Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic PainQian Wang0Jing Lin1Peng Yang2Yingye Liang3Dongming Lu4Kailong Wang5Wei Gan6Jianping Fu7Zhenbao Gan8Mingchen Ma9Pingting Wu10Fengshi He11Jun Pang12Hongliang Tang13Department of AcupunctureDepartment of AcupunctureGuangxi University of Chinese MedicineDepartment of MassageDepartment of MassageDepartment of MassageDepartment of MassageDepartment of MassageDepartment of MassageGuangxi University of Chinese MedicineGuangxi University of Chinese MedicineGuangxi University of Chinese MedicineDepartment of MassageDepartment of MassageThis study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided into 5 groups: the control group, sham-operated group, model group, sham massage group, and massage group. There were 8 rats in each group. SNL rat models were established in the model group, sham massage group, and massage group. Rats in the sham-operated group underwent surgery to expose the vertebral nerves, but no further procedures were performed. The control group consisted of intact animals. The rats in the massage group underwent massage using a massage simulation machine once a day for 14 d in succession; the hind limbs of the rats in the sham massage group were gently touched with a cloth bag once a day for 14 continuous days. The rats in the control group, the sham-operated group, and the model group did not receive any intervention and were observed for 14 d. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) of rats in each group were detected 1 d before modelling and at 1, 3, 7, and 14 d after modelling. Fourteen days after modelling, the expression levels of TLR4, IRAK1, TRAF6, TNF-α, and IL-6 were detected in all rats. The PWTL and PWMT of SNL rats were decreased, while these parameters were elevated after massage. SNL rats showed higher levels of TLR4, IRAK1, TRAF6, IL-6, and TNF-α, and massage effectively lowered the expression levels of these molecules. Inhibiting activation of the TLR4 signalling pathway, which can reduce the release of inflammatory factors, may be one mechanism by which massage treats neuropathic pain.http://dx.doi.org/10.1155/2020/8309745
collection DOAJ
language English
format Article
sources DOAJ
author Qian Wang
Jing Lin
Peng Yang
Yingye Liang
Dongming Lu
Kailong Wang
Wei Gan
Jianping Fu
Zhenbao Gan
Mingchen Ma
Pingting Wu
Fengshi He
Jun Pang
Hongliang Tang
spellingShingle Qian Wang
Jing Lin
Peng Yang
Yingye Liang
Dongming Lu
Kailong Wang
Wei Gan
Jianping Fu
Zhenbao Gan
Mingchen Ma
Pingting Wu
Fengshi He
Jun Pang
Hongliang Tang
Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
Pain Research and Management
author_facet Qian Wang
Jing Lin
Peng Yang
Yingye Liang
Dongming Lu
Kailong Wang
Wei Gan
Jianping Fu
Zhenbao Gan
Mingchen Ma
Pingting Wu
Fengshi He
Jun Pang
Hongliang Tang
author_sort Qian Wang
title Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
title_short Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
title_full Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
title_fullStr Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
title_full_unstemmed Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain
title_sort effect of massage on the tlr4 signalling pathway in rats with neuropathic pain
publisher Hindawi Limited
series Pain Research and Management
issn 1918-1523
publishDate 2020-01-01
description This study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided into 5 groups: the control group, sham-operated group, model group, sham massage group, and massage group. There were 8 rats in each group. SNL rat models were established in the model group, sham massage group, and massage group. Rats in the sham-operated group underwent surgery to expose the vertebral nerves, but no further procedures were performed. The control group consisted of intact animals. The rats in the massage group underwent massage using a massage simulation machine once a day for 14 d in succession; the hind limbs of the rats in the sham massage group were gently touched with a cloth bag once a day for 14 continuous days. The rats in the control group, the sham-operated group, and the model group did not receive any intervention and were observed for 14 d. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) of rats in each group were detected 1 d before modelling and at 1, 3, 7, and 14 d after modelling. Fourteen days after modelling, the expression levels of TLR4, IRAK1, TRAF6, TNF-α, and IL-6 were detected in all rats. The PWTL and PWMT of SNL rats were decreased, while these parameters were elevated after massage. SNL rats showed higher levels of TLR4, IRAK1, TRAF6, IL-6, and TNF-α, and massage effectively lowered the expression levels of these molecules. Inhibiting activation of the TLR4 signalling pathway, which can reduce the release of inflammatory factors, may be one mechanism by which massage treats neuropathic pain.
url http://dx.doi.org/10.1155/2020/8309745
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