Development of Single-Walled Carbon Nanotube-Based Biosensor for the Detection of Staphylococcus aureus

• Main Authors: Hyun-Kyung Choi, Jinyoung Lee, Mi-Kyung Park, Jun-Hyun Oh
• Format: Article
• Language: English
• Published: Hindawi-Wiley 2017-01-01
• Series: Journal of Food Quality
• Online Access: http://dx.doi.org/10.1155/2017/5239487

The goal of this research is to develop a single-walled carbon nanotube- (SWCNT-) based biosensor to detect Staphylococcus aureus. The specificity of 11 bacteria and polyclonal anti-Staphylococcus aureus antibodies (pAbs) was determined using an indirect ELISA. The pAbs were immobilized onto sensor platform after the hybridization of 1-pyrenebutanoic acid succinimidyl ester (PBASE). The resistance difference (ΔR) was calculated using a potentiostat. The bacteria detected by the biosensor were observed using a scanning electron microscope (SEM). The optimum concentration of SWCNTs on the platform was determined to be 0.1 mg/mL. The binding of pAbs with S. aureus resulted in a significant increase in resistance value of the biosensor (P<0.05). The SEM images confirmed the specific binding of S. aureus on the biosensor. The SWCNT-based biosensor was able to detect S. aureus with a limit of detection (LOD) of 4 log⁡CFU/mL.