Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

Comparative validation using quantitative real-time PCR (qPCR) and conventional PCR of bovine semen centrifuged in continuous density gradient

The objective of the present study was to determine the sperm enrichment with X-bearing spermatozoa, after one centrifugation in a Percoll or OptiPrep continuous density gradient, using quantitative real-time polymerase chain reaction (qPCR) of sperm DNA and resultant in vitro-produced bovine embryo...

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Bibliographic Details
Main Authors: M.V. Resende, A.C. Lucio, A.P. Perini, L.Z. Oliveira, A.O. Almeida, B.C.A. Alves, C.A. Moreira-Filho, I.W. Santos, V.F.M. Hossepian de Lima
Format: Article
Language:English
Published: Universidade Federal de Minas Gerais 2011-06-01
Series:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Subjects:
bovino
sexagem espermatozoides
centrifugação
sexagem embriões
qPCR
PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300002&lng=en&tlng=en

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