Development of Alternative Methods for Determining Soil Organic Matter

• Main Authors: Diego Mendes de Souza, Pedro Augusto de Oliveira Morais, Ivã Matsushige, Luismar Alves Rosa
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Ciência do Solo 2016-01-01
• Series: Revista Brasileira de Ciência do Solo
• Subjects: SOM; spectrophotometry; increase in functionality; response surface
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832016000100419&lng=en&tlng=en

ABSTRACT Soil organic matter (SOM) is important to fertility, since it performs several functions such as cycling, water and nutrient retention and soil aggregation, in addition to being an energy requirement for biological activity. This study proposes new trends to the Embrapa, Walkley-Black, and Mebius methods that allowed the determination of SOM by spectrophotometry, increasing functionality. The mass of 500 mg was reduced to 200 mg, generating a mean of 60 % saving of reagents and a decrease of 91 % in the volume of residue generated for the three methods without compromising accuracy and precision. We were able to optimize conditions for the Mebius method and establish the digestion time of maximum recovery of SOM by factorial design and response surface. The methods were validated by the estimate of figures of merits. Between the methods investigated, the optimized Mebius method was best suited for determining SOM, showing near 100 % recovery.