Novel Poly[(R)-3-Hydroxybutyrate]-Producing Bacterium Isolated from a Bolivian Hypersaline Lake

• Main Authors: María Soledad Marqués-Calvo, Miguel Miranda de Sousa Dias, Margarita Calafell, Alejandra Rodriguez-Contreras, Gerhart Braunegg, Martin Koller
• Format: Article
• Language: English
• Published: University of Zagreb 2013-01-01
• Series: Food Technology and Biotechnology
• Subjects: poly[(R)-3-hydroxybutyrate]; biopolymer; bioproduction; new strain; hypersaline environment; thermal properties
• Online Access: http://hrcak.srce.hr/file/146896

Poly[(R)-3-hydroxybutyrate] (PHB) constitutes a biopolymer synthesized from renewable resources by various microorganisms. This work focuses on finding a new PHB-producing bacterium capable of growing in conventional media used for industrial biopolymer production, its taxonomical identification, and characterization of its biopolymer. Thus, a bacterial isolation process was carried out from environmental samples of water and mud. Among the isolates, strain S29 was selected and used in a fed-batch fermentation to generate a biopolymer. This biopolymer was recovered and identified as PHB homopolymer. Surprisingly, it featured several fractions of different molecular masses, and thermal properties unusual for PHB. Hence, the microorganism S29, genetically identified as a new strain of Bacillus megaterium, proved to be interesting not only due to its growth and PHB accumulation kinetics under the investigated cultivation conditions, but also due to the thermal properties of the produced PHB.