EXPLORATION OF THE IMPACT OF THE EXTRACTION PARAMETERS ON BIOLOGICAL ACTIVE SUBSTANCES IN THE EXTRACTS FROM DRIED FRUIT BLACK BLUEBERRY (VACCINIUM MYRTILLUS L.)

• Main Authors: A. Georgieva, R. Stefanova, Kr. Krastev
• Format: Article
• Language: English
• Published: Trakia University 2020-03-01
• Series: Trakia Journal of Sciences
• Subjects: extraction parameters; extracts; biologically active substances; dried fruit black blueberry
• Online Access: http://tru.uni-sz.bg/tsj/Volume%2018,%202020,%20Number%201,%20Series%20Biomedical%20Sciences/A.Georgieva.pdf

The optimal parameters of the extraction process for the extraction of biologically active substances from the dried fruits of black blueberry have been established and experimentally confirmed. The phenolic complex of the extracts obtained at different parameters of the extraction process was investigated - selection of the extractant; extraction temperature; extraction time and hydromodule. Studied are the extracts obtained at the optimals extraction parameters for total phenols 7,34 g gallic acid / 100 g dry substance, phenolcarboxylic acids 0,61%, flavonoid phenolic compounds 194 mg%, sum anthocyanins 115 mg% and tannins 8,76 g catechin / 100g dry substance. On the basis of the experimental data, the technological regimes of the extraction process were selected. The use of 70% C2H5OH as an extractant is technologically warranted to produce extracts of dried fruits black blueberry with maximum content of phenols, flavonoids, anthocyanins and tannins. Technologically justified extraction temperature is 65-80оС. By increasing the extraction temperature to 80°C, the quantity of anthocyanins in the extracts increases, which positively affects the organoleptic evaluation of the final products. The optimum extraction time is 3-4 hours. During this extraction period, a maximum content of the phenolic complex is obtained. The results obtained suggest that the most advantageous hydromodul for extraction is 1:30.