| Summary: | Accurate detection of cadmium (Cd) and lead (Pb)-induced cross-stress on crops is essential for agricultural, ecological environment, and food security. The feasibility to diagnose and predict Cd−Pb cross-stress in agricultural soil was explored by measuring the visible and near-infrared reflectance of rice leaves. In this study, two models were developed—namely a diagnostic model and a prediction model. The diagnostic model was established based on visible and near-infrared reflectance spectroscopy (VNIRS) datasets with Support Vector Machine (SVM), followed by leave-one-out cross-validation (LOOCV). A partial least-squares (PLS) regression, as the prediction model was employed to predict the foliar concentration of Cd and Pb contents. To accurately calibrate the two models, a rigorous greenhouse experiment was designed and implemented, with 4 levels of treatments on each of the Cd and Pb stress on rice. Results show that with the appropriate pre-processing, the diagnostic model can identify 79% of Cd and 85% of Pb stress of any levels. The significant bands that have been used mainly distributed between 681−776 nm and 1224−1349 nm for Cd stress and 712−784 nm for Pb stress. The prediction model can estimate Cd with coefficient of determination of 0.7, but failed to predict Pb accurately. The results illustrated the feasibility to diagnose Cd stress accurately by measuring the visible and near-infrared reflectance of rice canopy in a cross-contamination soil environment. This study serves as one step forward to heavy metal pollutant detection in a farmland environment.
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