FRET Microscopy in Yeast
Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly i...
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MDPI AG
2019-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/9/4/122 |
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doaj-26c132e1b5214fc3859e4de89750c2232020-11-25T01:32:07ZengMDPI AGBiosensors2079-63742019-10-019412210.3390/bios9040122bios9040122FRET Microscopy in YeastMichal Skruzny0Emma Pohl1Marc Abella2Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, GermanyDepartment of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, GermanyDepartment of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, GermanyFörster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts <i>Saccharomyces cerevisiae</i> and <i>Schizosaccharomyces pombe</i>, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts.https://www.mdpi.com/2079-6374/9/4/122budding yeastfission yeastacceptor photobleachingsensitized emissionratiometric fretflimgfp |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Michal Skruzny Emma Pohl Marc Abella |
| spellingShingle |
Michal Skruzny Emma Pohl Marc Abella FRET Microscopy in Yeast Biosensors budding yeast fission yeast acceptor photobleaching sensitized emission ratiometric fret flim gfp |
| author_facet |
Michal Skruzny Emma Pohl Marc Abella |
| author_sort |
Michal Skruzny |
| title |
FRET Microscopy in Yeast |
| title_short |
FRET Microscopy in Yeast |
| title_full |
FRET Microscopy in Yeast |
| title_fullStr |
FRET Microscopy in Yeast |
| title_full_unstemmed |
FRET Microscopy in Yeast |
| title_sort |
fret microscopy in yeast |
| publisher |
MDPI AG |
| series |
Biosensors |
| issn |
2079-6374 |
| publishDate |
2019-10-01 |
| description |
Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts <i>Saccharomyces cerevisiae</i> and <i>Schizosaccharomyces pombe</i>, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts. |
| topic |
budding yeast fission yeast acceptor photobleaching sensitized emission ratiometric fret flim gfp |
| url |
https://www.mdpi.com/2079-6374/9/4/122 |
| work_keys_str_mv |
AT michalskruzny fretmicroscopyinyeast AT emmapohl fretmicroscopyinyeast AT marcabella fretmicroscopyinyeast |
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1725083214001733632 |