Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies
A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and <i>Ixodes ricinus</i> tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulenc...
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MDPI AG
2020-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/8/902 |
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doaj-26b04d569bc84d10bebb63c031711cc82020-11-25T03:01:31ZengMDPI AGViruses1999-49152020-08-011290290210.3390/v12080902Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of QuasispeciesRenata Helmová0Václav Hönig1Hana Tykalová2Martin Palus3Lesley Bell-Sakyi4Libor Grubhoffer5Faculty of Science, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech RepublicFaculty of Science, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech RepublicFaculty of Science, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech RepublicInstitute of Parasitology, Biology Centre of the Czech Academy of Sciences, 370 05 České Budějovice, Czech RepublicInstitute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UKFaculty of Science, University of South Bohemia in České Budějovice, 37005 České Budějovice, Czech RepublicA highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and <i>Ixodes ricinus</i> tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.https://www.mdpi.com/1999-4915/12/8/902TBEVhost alternationneuroinvasivenessgenome mutationquasispeciesflavivirus adaptation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Renata Helmová Václav Hönig Hana Tykalová Martin Palus Lesley Bell-Sakyi Libor Grubhoffer |
| spellingShingle |
Renata Helmová Václav Hönig Hana Tykalová Martin Palus Lesley Bell-Sakyi Libor Grubhoffer Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies Viruses TBEV host alternation neuroinvasiveness genome mutation quasispecies flavivirus adaptation |
| author_facet |
Renata Helmová Václav Hönig Hana Tykalová Martin Palus Lesley Bell-Sakyi Libor Grubhoffer |
| author_sort |
Renata Helmová |
| title |
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies |
| title_short |
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies |
| title_full |
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies |
| title_fullStr |
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies |
| title_full_unstemmed |
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies |
| title_sort |
tick-borne encephalitis virus adaptation in different host environments and existence of quasispecies |
| publisher |
MDPI AG |
| series |
Viruses |
| issn |
1999-4915 |
| publishDate |
2020-08-01 |
| description |
A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) and <i>Ixodes ricinus</i> tick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals. |
| topic |
TBEV host alternation neuroinvasiveness genome mutation quasispecies flavivirus adaptation |
| url |
https://www.mdpi.com/1999-4915/12/8/902 |
| work_keys_str_mv |
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