A simple method for DNA isolation from Xanthomonas spp.

A simple method for DNA isolation from Xanthomonas spp.

A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The am...

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Main Authors: Gomes Luiz Humberto, Duarte Keila Maria Roncato, Andrino Felipe Gabriel, Tavares Flavio Cesar Almeida
Format: Article
Language:English
Published: Universidade de São Paulo 2000-01-01
Series:Scientia Agricola
Subjects:
DNA
Xanthomonas
RAPD
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
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spelling doaj-2697f2ad757c41a39d204d9a6b5d24642020-11-24T20:43:53ZengUniversidade de São PauloScientia Agricola0103-90161678-992X2000-01-01573553555A simple method for DNA isolation from Xanthomonas spp.Gomes Luiz HumbertoDuarte Keila Maria RoncatoAndrino Felipe GabrielTavares Flavio Cesar AlmeidaA simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028DNAXanthomonasRAPD
collection DOAJ
language English
format Article
sources DOAJ
author Gomes Luiz Humberto
Duarte Keila Maria Roncato
Andrino Felipe Gabriel
Tavares Flavio Cesar Almeida
spellingShingle Gomes Luiz Humberto
Duarte Keila Maria Roncato
Andrino Felipe Gabriel
Tavares Flavio Cesar Almeida
A simple method for DNA isolation from Xanthomonas spp.
Scientia Agricola
DNA
Xanthomonas
RAPD
author_facet Gomes Luiz Humberto
Duarte Keila Maria Roncato
Andrino Felipe Gabriel
Tavares Flavio Cesar Almeida
author_sort Gomes Luiz Humberto
title A simple method for DNA isolation from Xanthomonas spp.
title_short A simple method for DNA isolation from Xanthomonas spp.
title_full A simple method for DNA isolation from Xanthomonas spp.
title_fullStr A simple method for DNA isolation from Xanthomonas spp.
title_full_unstemmed A simple method for DNA isolation from Xanthomonas spp.
title_sort simple method for dna isolation from xanthomonas spp.
publisher Universidade de São Paulo
series Scientia Agricola
issn 0103-9016
1678-992X
publishDate 2000-01-01
description A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.
topic DNA
Xanthomonas
RAPD
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
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