Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production

<abstract language="eng">Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil. The cyclodextrins (CDs) production by CGTases was evaluated using starches from different sources. CDs wer...

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Bibliographic Details
Main Authors: Cristiane Moriwaki, Cassiana Mazzer, Rúbia Pazzetto, Graciette Matioli
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2009-01-01
Series:Química Nova
Subjects:
cyclodextrin
CGTase
biospecific affinity chromatography
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000900024
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spelling doaj-267d1a39d8b247e6897724fe764497a92020-11-24T23:00:37ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642009-01-013292360236610.1590/S0100-40422009000900024Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins productionCristiane MoriwakiCassiana MazzerRúbia PazzettoGraciette Matioli<abstract language="eng">Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil. The cyclodextrins (CDs) production by CGTases was evaluated using starches from different sources. CDs were measured through the Complexation Theory and by HPLC. CGTase from Bacillus firmus strain 7B showed the best production (30 mmol/L of &#946;-CD and 4.3 mmol/L of &#947;-CD), and its cultivation conditions were optimized. The maximum enzymatic activity was achieved using lung peptone, soluble starch and agitation speed of 160 rpm. Studied CGTases were shown quite interesting for the industrial production of CDs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000900024cyclodextrinCGTasebiospecific affinity chromatography
collection DOAJ
language English
format Article
sources DOAJ
author Cristiane Moriwaki
Cassiana Mazzer
Rúbia Pazzetto
Graciette Matioli
spellingShingle Cristiane Moriwaki
Cassiana Mazzer
Rúbia Pazzetto
Graciette Matioli
Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
Química Nova
cyclodextrin
CGTase
biospecific affinity chromatography
author_facet Cristiane Moriwaki
Cassiana Mazzer
Rúbia Pazzetto
Graciette Matioli
author_sort Cristiane Moriwaki
title Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
title_short Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
title_full Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
title_fullStr Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
title_full_unstemmed Produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas Production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
title_sort produção, purificação e aumento da performance de ciclodextrina glicosiltransferases para produção de ciclodextrinas production, purification and performance improve of cyclodextrin glycosyl transferases to cyclodextrins production
publisher Sociedade Brasileira de Química
series Química Nova
issn 0100-4042
1678-7064
publishDate 2009-01-01
description <abstract language="eng">Biospecific affinity chromatography was used to purify three cyclodextrin glycosyl transferases (CGTases) obtained from microorganisms isolated of soil. The cyclodextrins (CDs) production by CGTases was evaluated using starches from different sources. CDs were measured through the Complexation Theory and by HPLC. CGTase from Bacillus firmus strain 7B showed the best production (30 mmol/L of &#946;-CD and 4.3 mmol/L of &#947;-CD), and its cultivation conditions were optimized. The maximum enzymatic activity was achieved using lung peptone, soluble starch and agitation speed of 160 rpm. Studied CGTases were shown quite interesting for the industrial production of CDs.
topic cyclodextrin
CGTase
biospecific affinity chromatography
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422009000900024
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AT cassianamazzer producaopurificacaoeaumentodaperformancedeciclodextrinaglicosiltransferasesparaproducaodeciclodextrinasproductionpurificationandperformanceimproveofcyclodextringlycosyltransferasestocyclodextrinsproduction
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