Microsatellite DNA-markers in the study of the gene pool of fruit crops

• Main Authors: Suprun Ivan, Tokmakov Sergei, Lobodina Elena
• Format: Article
• Language: English
• Published: EDP Sciences 2020-01-01
• Series: BIO Web of Conferences
• Online Access: https://www.bio-conferences.org/articles/bioconf/full_html/2020/09/bioconf_bioengineering2020_03001/bioconf_bioengineering2020_03001.html

This article describes development of multilocus SSR-markers sets for genotyping Pyrus, Prunus, and Malus from various genetic collections of the South of Russia. Generated multiplex sets of SSR-markers were used in the certification of cultivated varieties and in the analysis of the genetic structure of Pyrus, Prunus and Malus species collections. The results of SSR genotyping of pear, apple, plum and sweet cherry made it possible to establish genetic relationships between varieties, including groups of modern varieties of Russian and foreign breeding and, in turn, local autochthonous varieties. In general, the use of these multiplexes has confirmed their effectiveness in solving the assigned tasks.