Co-Regulated Expression of TGF-β Variants and miR-21 In Bladder Cancer

• Main Authors: Hamideh Monfared, Seyed Amir Mohsen Ziaee, Mahmoud Hashemitabar, Hamid Khayatzadeh, Vahid Kheyrollahi, Mahmood Tavallaei, Seyed Javad Mowla
• Format: Article
• Language: English
• Published: Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences 2013-09-01
• Series: Urology Journal
• Subjects: biological markers; analysis; urinary bladder neoplasms; miR-21; TGF-β; gene expression profiling
• Online Access: http://www.urologyjournal.org/index.php/uj/article/view/1487/752
• ISSN: 1735-1308; 1735-546X

Purpose: To investigate a potential alteration in the expression levels of transforminggrowth factor β (TGF-β) and miR-21 in bladder cancer tissues.Material and Methods: Using real-time polymerase chain reaction (PCR) method, weexamined a potential correlated expression of miR-21 and TGF-β variants in 30 bladdertumors and their marginal/non-tumor biopsy specimens obtained from the same patients.Results: Our data revealed a significant down-regulation of TGF-β variants (P = .03) alongwith a non-significant alteration in the expression of miR-21 in tumor vs. non-tumor samples.However, in contrast to low-grade tumors, the expression of miR-21 was upregulatedin high-grade ones, and the expression level can efficiently discriminate low-grade tumorsfrom high-grade ones (P = .03).Conclusion: In accordance to the observed similarity between TGF-β variants and miR-21gene expression alterations in bladder tumors, treating 5637 bladder cancer cell line withTGF-β recombinant protein caused a significant upregulation of miR-21. The later findingfurther confirmed a correlated expression of TGF-β and miR-21 in bladder tumors.