Data on the uptake of CpG-loaded amino-dextran nanoparticles by antigen-presenting cells

• Main Authors: Hien V. Nguyen, Katrin Campbell, Gavin F. Painter, Sarah L. Young, Greg F. Walker
• Format: Article
• Language: English
• Published: Elsevier 2021-04-01
• Series: Data in Brief
• Subjects: Dextran nanoparticle; CpG oligonucleotides; Electrostatic adsorption; Covalent conjugation; Flow cytometry; Fluorescence microscopy
• Online Access: http://www.sciencedirect.com/science/article/pii/S2352340921001670

Cytosine-phosphate-guanine (CpG) oligonucleotides are commonly-used vaccine adjuvants to promote the activation of antigen-presenting cells (APCs). To mount an effective immune response, CpG needs to be internalized and bind to its endosomal Toll-like receptor 9 (TLR-9) inside the APCs. Using flow cytometry and fluorescence microscopy, this article presents the cellular uptake data of the amino-dextran nanoparticle (aDNP) and aDNP loaded with CpG immobilized on its surface by either electrostatic adsorption or covalent conjugation. The uptake of fluorescently-labelled aDNPs by murine splenic dendritic cells and macrophages was determined by flow cytometry and uptake by murine bone-marrow-derived dendritic cells was evaluated by fluorescence microscopy. The data presented in this paper correlates with the in vitro immune-stimulatory activity observed for the two different CpG loading methods in the research article “Nanoparticle system based on amino-dextran as a drug delivery vehicle: immune-stimulatory CpG-oligonucleotide loading and delivery” (Nguyen et al., 2020) [1]. The data provide experimental evidence for a better understanding how the nanoparticle surface loading method of CpG influences the uptake of these nanoparticles by antigen-presenting cells as a step guide in the design of more effective vaccine formulations.